Optimization of Cat’s Whiskers Tea (Orthosiphon stamineus) Using Supercritical Carbon Dioxide and Selective Chemotherapeutic Potential against Prostate Cancer Cells
Joint Authors
Mohd Omar, A. K.
Abdul Majid, A. M. S.
Al-Suede, Fouad Saleih R.
Abdul Majid, Aman S.
Baharetha, Hussin M.
Hassan, Loiy E. A.
Nassar, Zeyad D.
Ahamed, Mohamed B. Khadeer
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-07
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Cat’s whiskers (Orthosiphon stamineus) leaves extracts were prepared using supercritical CO2 (SC-CO2) with full factorial design to determine the optimum extraction parameters.
Nine extracts were obtained by varying pressure, temperature, and time.
The extracts were analysed using FTIR, UV-Vis, and GC-MS.
Cytotoxicity of the extracts was evaluated on human (colorectal, breast, and prostate) cancer and normal fibroblast cells.
Moderate pressure (31.1 MPa) and temperature (60°C) were recorded as optimum extraction conditions with high yield (1.74%) of the extract (B2) at 60 min extraction time.
The optimized extract (B2) displayed selective cytotoxicity against prostate cancer (PC3) cells (IC50 28 µg/mL) and significant antioxidant activity (IC50 42.8 µg/mL).
Elevated levels of caspases 3/7 and 9 in B2-treated PC3 cells suggest the induction of apoptosis through nuclear and mitochondrial pathways.
Hoechst and rhodamine assays confirmed the nuclear condensation and disruption of mitochondrial membrane potential in the cells.
B2 also demonstrated inhibitory effects on motility and colonies of PC3 cells at its subcytotoxic concentrations.
It is noteworthy that B2 displayed negligible toxicity against the normal cells.
Chemometric analysis revealed high content of essential oils, hydrocarbon, fatty acids, esters, and aromatic sesquiterpenes in B2.
This study highlights the therapeutic potentials of SC-CO2 extract of cat’s whiskers in targeting prostate carcinoma.
American Psychological Association (APA)
Al-Suede, Fouad Saleih R.& Ahamed, Mohamed B. Khadeer& Abdul Majid, Aman S.& Baharetha, Hussin M.& Hassan, Loiy E. A.& Mohd Omar, A. K.…[et al.]. 2014. Optimization of Cat’s Whiskers Tea (Orthosiphon stamineus) Using Supercritical Carbon Dioxide and Selective Chemotherapeutic Potential against Prostate Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1035182
Modern Language Association (MLA)
Al-Suede, Fouad Saleih R.…[et al.]. Optimization of Cat’s Whiskers Tea (Orthosiphon stamineus) Using Supercritical Carbon Dioxide and Selective Chemotherapeutic Potential against Prostate Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1035182
American Medical Association (AMA)
Al-Suede, Fouad Saleih R.& Ahamed, Mohamed B. Khadeer& Abdul Majid, Aman S.& Baharetha, Hussin M.& Hassan, Loiy E. A.& Mohd Omar, A. K.…[et al.]. Optimization of Cat’s Whiskers Tea (Orthosiphon stamineus) Using Supercritical Carbon Dioxide and Selective Chemotherapeutic Potential against Prostate Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1035182
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035182