Effects of Modified Simiao Decoction on IL-1β and TNFα Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation
Joint Authors
Hu, Yonghong
Liu, Yafei
Chen, Zhe
Tu, Shenghao
Wang, Yu
Dong, Hui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Simiao pill, a Chinese herbal formula containing four herbs, has been used in the treatment of gouty arthritis for many years.
The aim of this study was to explore the effects of modified Simiao decoction (MSD) on IL-1β and TNFα secretion in monocytic THP-1 cells with monosodium urate (MSU) crystals-induced inflammation.
The MSU crystals-induced inflammation model in THP-1 cells was successfully established by the stimulation of phorbol 12-myristate 13-acetate (PMA) and MSU crystals.
Then, the MSD-derived serum or control serum extracted from rat was administered to different treatment groups.
The morphology of MSU crystals and THP-1 cells was observed.
IL-1β and TNFα protein expression in supernatant of THP-1 cells were determined by ELISA.
Our data demonstrated that MSU crystals induced time-dependent increase of IL-1β and TNFα.
Moreover, MSD significantly decreased IL-1β release in THP-1 cells with MSU crystals-induced inflammation.
These results suggest that MSD is promising in the treatment of MSU crystals-induced inflammation in THP-1 cells.
MSD may act as an anti-IL-1 agent in treating gout.
The underlying mechanism may be related to NALP3 inflammasome which needs to be validated in future studies.
American Psychological Association (APA)
Liu, Yafei& Tu, Shenghao& Chen, Zhe& Wang, Yu& Hu, Yonghong& Dong, Hui. 2014. Effects of Modified Simiao Decoction on IL-1β and TNFα Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1035187
Modern Language Association (MLA)
Liu, Yafei…[et al.]. Effects of Modified Simiao Decoction on IL-1β and TNFα Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1035187
American Medical Association (AMA)
Liu, Yafei& Tu, Shenghao& Chen, Zhe& Wang, Yu& Hu, Yonghong& Dong, Hui. Effects of Modified Simiao Decoction on IL-1β and TNFα Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1035187
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035187