Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose
Joint Authors
Yan, Rui
Qu, Longmei
Luo, Nan
Liu, Yang
Liu, Yu
Li, Li
Chen, Lijiang
Source
International Journal of Analytical Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-03
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Lactose is a widely used pharmaceutical excipient, sometimes irreplaceable.
Traces of residual proteins left during production of lactose are potential allergen to body.
The present paper describes a sensitive and specific LC-MS method for the determination of α-lactalbumin (α-La) in lactose samples.
Chromatographic separation was performed on an Acquity UPLC BEH300 C18 column ( 2.1 × 150 mm, 1.7 μm) with an isocratic mobile phase consisting of water containing 0.1% TFA and acetonitrile containing 0.1% TFA (80 : 20, v/v).
Mass spectrometric detection was achieved by a triple-quadrupole mass spectrometer equipped with an ESI interface operating in positive ionization mode.
Quantitation was performed using selected ion monitoring of m/z 2364 for α-La.
The calibration curve was linear from 0.2 to 10 µg/mL.
The intra- and interday precisions were less than 7.6% and the accuracy ranged from 96.4 to 104.5%.
The limit of quantification (LOQ) was 0.15 µg/mL and the limit of detection (LOD) was 0.05 µg/mL.
This method was then successfully applied to investigate 6 different lactose samples.
The application can provide technical preparation for the development of specification of lactose.
American Psychological Association (APA)
Yan, Rui& Qu, Longmei& Luo, Nan& Liu, Yang& Liu, Yu& Li, Li…[et al.]. 2014. Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose. International Journal of Analytical Chemistry،Vol. 2014, no. 2014, pp.1-4.
https://search.emarefa.net/detail/BIM-1035936
Modern Language Association (MLA)
Yan, Rui…[et al.]. Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose. International Journal of Analytical Chemistry No. 2014 (2014), pp.1-4.
https://search.emarefa.net/detail/BIM-1035936
American Medical Association (AMA)
Yan, Rui& Qu, Longmei& Luo, Nan& Liu, Yang& Liu, Yu& Li, Li…[et al.]. Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose. International Journal of Analytical Chemistry. 2014. Vol. 2014, no. 2014, pp.1-4.
https://search.emarefa.net/detail/BIM-1035936
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035936