Effect of Serum and Oxygen Concentration on Gene Expression and Secretion of Paracrine Factors by Mesenchymal Stem Cells
Joint Authors
Page, Patrick
DeJong, Joshua
Bandstra, Alaina
Boomsma, Robert A.
Source
International Journal of Cell Biology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Mesenchymal stem cells (MSC) secrete paracrine factors that may exert a protective effect on the heart after coronary artery occlusion.
This study was done to determine the effect of hypoxia and serum levels on the mRNA expression and secretion of paracrine factors.
Mouse bone marrow MSC were cultured with 5% or 20% serum and in either normoxic (21% O2) or hypoxic (1% O2) conditions.
Expression of mRNA for vascular endothelial growth factor (VEGF), monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1α (MIP-1α), MIP-1β, and matrix metalloproteinase-2 (MMP-2) was determined by RT-qPCR.
Secretion into the culture media was determined by ELISA.
Hypoxia caused a reduction in gene expression for MCP-1 and an increase for VEGF (5% serum), MIP-1α, MIP-1β, and MMP-2.
Serum reduction lowered gene expression for VEGF (normoxia), MCP-1 (hypoxia), MIP-1α (hypoxia), MIP-1β (hypoxia), and MMP-2 (hypoxia) and increased gene expression for MMP-2 (normoxia).
The level of secretion of these factors into the media generally paralleled gene expression with some exceptions.
These data demonstrate that serum and oxygen levels have a significant effect on the gene expression and secretion of paracrine factors by MSC which will affect how MSC interact in vivo during myocardial ischemia.
American Psychological Association (APA)
Page, Patrick& DeJong, Joshua& Bandstra, Alaina& Boomsma, Robert A.. 2014. Effect of Serum and Oxygen Concentration on Gene Expression and Secretion of Paracrine Factors by Mesenchymal Stem Cells. International Journal of Cell Biology،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1036350
Modern Language Association (MLA)
Page, Patrick…[et al.]. Effect of Serum and Oxygen Concentration on Gene Expression and Secretion of Paracrine Factors by Mesenchymal Stem Cells. International Journal of Cell Biology No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1036350
American Medical Association (AMA)
Page, Patrick& DeJong, Joshua& Bandstra, Alaina& Boomsma, Robert A.. Effect of Serum and Oxygen Concentration on Gene Expression and Secretion of Paracrine Factors by Mesenchymal Stem Cells. International Journal of Cell Biology. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1036350
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036350