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Determination of β-Cyano-L-alanine, γ-Glutamyl- β-cyano-L-alanine, and Common Free Amino Acids in Vicia sativa (Fabaceae) Seeds by Reversed-Phase High-Performance Liquid Chromatography
Joint Authors
Megías, Cristina
Cortés-Giraldo, Isabel
Girón-Calle, Julio
Alaiz, Manuel
Vioque Peña, Javier
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-22
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A method for determination of β-cyano-L-alanine, γ-glutamyl-β-cyano-L-alanine and other free amino acids in Vicia sativa is presented.
Seed extracts were derivatized by reaction with diethyl ethoxymethylenemalonate and analyzed by reverse-phase high-performance liquid chromatography.
Calibration curves showed very good linearity of the response.
The limit of detection and quantification was 0.15 and 0.50 μM, respectively.
The method has high intra- (RSD = 0.28–0.31%) and interrepeatability (RSD = 2.76–3.08%) and remarkable accuracy with a 99% recovery in spiked samples.
The method is very easy to carry out and allows for ready analysis of large number of samples using very basic HPLC equipment because the derivatized samples are very stable and have very good chromatographic properties.
The method has been applied to the determination of γ-glutamyl-β-cyano-L-alanine, β-cyano-L-alanine, and common free amino acids in eight wild populations of V.
sativa from southwestern Spain.
American Psychological Association (APA)
Megías, Cristina& Cortés-Giraldo, Isabel& Girón-Calle, Julio& Vioque Peña, Javier& Alaiz, Manuel. 2014. Determination of β-Cyano-L-alanine, γ-Glutamyl- β-cyano-L-alanine, and Common Free Amino Acids in Vicia sativa (Fabaceae) Seeds by Reversed-Phase High-Performance Liquid Chromatography. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1039823
Modern Language Association (MLA)
Megías, Cristina…[et al.]. Determination of β-Cyano-L-alanine, γ-Glutamyl- β-cyano-L-alanine, and Common Free Amino Acids in Vicia sativa (Fabaceae) Seeds by Reversed-Phase High-Performance Liquid Chromatography. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1039823
American Medical Association (AMA)
Megías, Cristina& Cortés-Giraldo, Isabel& Girón-Calle, Julio& Vioque Peña, Javier& Alaiz, Manuel. Determination of β-Cyano-L-alanine, γ-Glutamyl- β-cyano-L-alanine, and Common Free Amino Acids in Vicia sativa (Fabaceae) Seeds by Reversed-Phase High-Performance Liquid Chromatography. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1039823
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1039823