The Effect of TLR9 Agonist CpG Oligodeoxynucleotides on the Intestinal Immune Response of Cobia (Rachycentron canadum)
Joint Authors
Cheng, Ta Chih
Byadgi, Omkar
Puteri, Dinda
Lee, Jai-Wei
Chang, Tsung-Chou
Lee, Yan-Horn
Chu, Chun-Yen
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-02
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Cytosine-guanine oligodeoxynucleotide (CpG ODN) motifs of bacterial DNA are recognized through toll-like receptor 9 (TLR9) and are potent activators of innate immunity.
However, the interaction between TLR9 and CpG ODN in aquatic species has not been well characterized.
Hence, cobia TLR9 isoform B (RCTLR9B) was cloned and its expression and induction in intestine were investigated.
RCTLR9B cDNA consists of 3113bp encoding 1009 amino acids containing three regions, leucine rich repeats, transmembrane domain, and toll/interleukin-1 receptor (TIR) domain.
Intraperitoneal injection of CpG ODN 2395 upregulated RCTLR9 A and B and MyD88 and also induced the expressions of Mx, chemokine CC, and interleukin IL-1β.
Cobia intraperitoneally injected with CpG ODN 1668 and 2395 had increased survival rates after challenge with Photobacterium damselae subsp.
piscicida.
In addition, formulation of CpG ODN with formalin-killed bacteria (FKB) and aluminum hydroxide gel significantly increased expressions of RCTLR9 A (50 folds) and B (30 folds) isoforms at 10 dpi (CpG ODN 1668) and MyD88 (21 folds) at 6 dpv (CpG ODN 2395).
Subsequently, IL-1β increased at 6 dpv in 1668 group.
No histopathological damage and inflammatory responses were observed in the injected cobia.
Altogether, these results facilitate CpG ODNs as an adjuvant to increase bacterial disease resistance and efficacy of vaccines in cobia.
American Psychological Association (APA)
Byadgi, Omkar& Puteri, Dinda& Lee, Jai-Wei& Chang, Tsung-Chou& Lee, Yan-Horn& Chu, Chun-Yen…[et al.]. 2014. The Effect of TLR9 Agonist CpG Oligodeoxynucleotides on the Intestinal Immune Response of Cobia (Rachycentron canadum). Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1040809
Modern Language Association (MLA)
Byadgi, Omkar…[et al.]. The Effect of TLR9 Agonist CpG Oligodeoxynucleotides on the Intestinal Immune Response of Cobia (Rachycentron canadum). Journal of Immunology Research No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1040809
American Medical Association (AMA)
Byadgi, Omkar& Puteri, Dinda& Lee, Jai-Wei& Chang, Tsung-Chou& Lee, Yan-Horn& Chu, Chun-Yen…[et al.]. The Effect of TLR9 Agonist CpG Oligodeoxynucleotides on the Intestinal Immune Response of Cobia (Rachycentron canadum). Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1040809
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040809