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IFN-γ-Secreting-Mesenchymal Stem Cells Exert an Antitumor Effect In Vivo via the TRAIL Pathway
Joint Authors
Yang, Xinyuan
Du, Jingchun
Xu, Xia
Xu, Chun
Song, Wu
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Mesenchymal stem cells (MSCs) can exhibit either prooncogenic or antitumor properties depending on the context.
Based on our previous study, we hypothesized that MSCs engineered to deliver IFN-γ would kill cancer cells through persistent activation of the TRAIL pathway.
Human bone-marrow (BM-) derived MSCs were isolated, amplified, and transduced with a lentiviral vector encoding the IFN-γ gene under the control of the EF1α promoter.
The IFN-γ-modified MSCs effectively secreted functional IFN-γ, which led to long-term expression of TRAIL.
More importantly, the IFN-γ-modified MSCs selectively induced apoptosis in lung tumor cells through caspase-3 activation within the target cells.
The percentage of activated-caspase-3-positive tumor cells in IFN-γ-modified MSCs cocultures was significantly higher than in control MSCs cocultures.
Treatment with anti-TRAIL antibody dramatically suppressed the caspase-3 activation observed in H460 cells.
After injection into nude mice, the IFN-γ-modified MSCs inhibited the growth and progression of lung carcinoma compared with control cells.
Collectively, our results provide a new strategy for tumor therapy that utilizes IFN-γ-modified MSCs.
American Psychological Association (APA)
Yang, Xinyuan& Du, Jingchun& Xu, Xia& Xu, Chun& Song, Wu. 2014. IFN-γ-Secreting-Mesenchymal Stem Cells Exert an Antitumor Effect In Vivo via the TRAIL Pathway. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040836
Modern Language Association (MLA)
Yang, Xinyuan…[et al.]. IFN-γ-Secreting-Mesenchymal Stem Cells Exert an Antitumor Effect In Vivo via the TRAIL Pathway. Journal of Immunology Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1040836
American Medical Association (AMA)
Yang, Xinyuan& Du, Jingchun& Xu, Xia& Xu, Chun& Song, Wu. IFN-γ-Secreting-Mesenchymal Stem Cells Exert an Antitumor Effect In Vivo via the TRAIL Pathway. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040836
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040836