Human CD38hiCD138+ Plasma Cells Can Be Generated In Vitro from CD40-Activated Switched-Memory B Lymphocytes
Joint Authors
Itoua Maïga, Rayelle
Bonnaure, Guillaume
Tremblay Rochette, Josiane
Néron, Sonia
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-23
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
B lymphocyte differentiation into long-lived plasma cells is the keystone event for the production of long-term protective antibodies.
CD40-CD154 and CD27-CD70 interactions are involved in human B lymphocyte differentiation into CD 38 hi CD 138 + cells in vivo as well as in vitro.
In this study, we have compared these interactions in their capacity to drive switched-memory B lymphocytes differentiation into CD 38 hi CD 138 + plasma cells.
The targeted B lymphocytes were isolated from human peripheral blood, expanded for 19 days, and then submitted to CD70 or CD154 interactions for 14 days.
The expanded B lymphocytes were constitutively expressing CD39, whereas CD31’s expression was noticed only following the in vitro differentiation step (day 5) and was exclusively present on the CD 38 hi cell population.
Furthermore, the generated CD 38 hi CD 138 + cells showed a higher proportion of CD31+ cells than the CD 38 hi CD 138 - cells.
Besides, analyses done with human blood and bone marrow plasma cells showed that in vivo and de novo generated CD 38 hi CD 138 + cells have a similar CD31 expression profile but are distinct according to their reduced CD39 expression level.
Overall, we have evidences that in vitro generated plasma cells are heterogeneous and appear as CD39+ precursors to the ones present in bone marrow niches.
American Psychological Association (APA)
Itoua Maïga, Rayelle& Bonnaure, Guillaume& Tremblay Rochette, Josiane& Néron, Sonia. 2014. Human CD38hiCD138+ Plasma Cells Can Be Generated In Vitro from CD40-Activated Switched-Memory B Lymphocytes. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1040929
Modern Language Association (MLA)
Itoua Maïga, Rayelle…[et al.]. Human CD38hiCD138+ Plasma Cells Can Be Generated In Vitro from CD40-Activated Switched-Memory B Lymphocytes. Journal of Immunology Research No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1040929
American Medical Association (AMA)
Itoua Maïga, Rayelle& Bonnaure, Guillaume& Tremblay Rochette, Josiane& Néron, Sonia. Human CD38hiCD138+ Plasma Cells Can Be Generated In Vitro from CD40-Activated Switched-Memory B Lymphocytes. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1040929
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040929