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Reliability of a Fully Automated Interpretation of γ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions
Joint Authors
Heydenreich, Juliane
Otto, Christoph
Mayer, Frank
Carlsohn, Anja
Source
Journal of Nutrition and Metabolism
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Analysis of γ-H2AX foci is a promising approach to evaluate exercise-induced DNA damage.
However, baseline levels and day-to-day variability of γ-H2AX foci have not been investigated in healthy subjects at rest.
Methods.
Blood was taken from eight moderately trained healthy males (29 ± 3 yrs, 1.84 ± 0.03 m, and 85 ± 6 kg) at two separate days (M1/M2) after 24-hour exercise cessation.
Number of γ-H2AX foci per 100 lymphocytes (N), number of foci per affected lymphocyte (NAL), percentage of affected lymphocytes (PAL), and diameter (D) of γ-H2AX foci were analyzed (mean ± SD).
Differences between M1 and M2 were analyzed using paired t-tests (α = 0.05).
Day-to-day variability was evaluated by calculating the coefficients of variation (CV%), bias, and limits of agreement (LoA).
Results.
There were no statistically significant differences between M1 (N: 7.6 ± 4.4, NAL: 1.2 ± 0.2, PAL: 5.9 ± 2.6%, and D: 0.63 ± 0.07) and M2 (N: 8.4 ± 4.6, NAL: 1.3 ± 0.1, PAL: 6.9 ± 4.2%, and D: 0.66 ± 0.06).
CV was calculated to be 98.5% (N), 88.9% (PAL), 11.3% (NAL), and 8.0% (D).
Bias (LoA) was 0.75 (−15.2/13.7), −0.02 (−0.36/0.33), −1.0 (−11.9/9.9), and −0.04 (−0.16/0.09), respectively.
Conclusions.
Background level in healthy subjects is approximately 0.07 to 0.09 γ-H2AX foci/cell.
NAL and D are reliable measures.
American Psychological Association (APA)
Heydenreich, Juliane& Otto, Christoph& Mayer, Frank& Carlsohn, Anja. 2014. Reliability of a Fully Automated Interpretation of γ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions. Journal of Nutrition and Metabolism،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1042161
Modern Language Association (MLA)
Heydenreich, Juliane…[et al.]. Reliability of a Fully Automated Interpretation of γ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions. Journal of Nutrition and Metabolism No. 2014 (Dec. 2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1042161
American Medical Association (AMA)
Heydenreich, Juliane& Otto, Christoph& Mayer, Frank& Carlsohn, Anja. Reliability of a Fully Automated Interpretation of γ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions. Journal of Nutrition and Metabolism. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1042161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1042161