Tumor Necrosis Factor-α-Induced Colitis Increases NADPH Oxidase 1 Expression, Oxidative Stress, and Neutrophil Recruitment in the Colon: Preventive Effect of Apocynin

Joint Authors

Mouzaoui, Souad
Djerdjouri, Bahia
Makhezer, Nesrine
Kroviarski, Yolande
El Benna, Jamel
Dang, Pham My-Chan

Source

Mediators of Inflammation

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-09-03

Country of Publication

Egypt

No. of Pages

15

Main Subjects

Diseases

Abstract EN

Reactive oxygen species- (ROS-) mediated injury has been implicated in several inflammatory disorders, including inflammatory bowel disease (IBD).

NADPH oxidases (NOXs) are the major source of endogenous ROS.

Here, we investigated the role of NOXs derived-ROS in a mouse model of colitis induced by the proinflammatory cytokine, tumor necrosis factor-α (TNF-α).

Intraperitoneal injection of TNFα (10 μg · kg−1) induced an acute inflammation of the colon and a marked increase in expression of NADPH oxidase 1 (NOX1), a colon specific NADPH oxidase isoform.

TNFα-induced colitis was also characterized by high production of keratinocyte-derived chemokine (KC) and mucosal infiltration of neutrophils, NOX2-expressing cells.

Concomitantly, ROS production and lipid peroxidation were significantly enhanced while catalase activity and glutathione level were reduced indicating a redox imbalance in the colon.

Furthermore, the redox-sensitive MAP kinases, ERK1/2 and p38 MAPK, were activated during TNFα-induced colitis.

Pretreatment of mice with apocynin, an NADPH oxidase inhibitor with antioxidant properties, before TNFα challenge, prevented all these events.

These data suggest that ROS derived from NADPH oxidases (mainly NOX1 and NOX2) and MAP kinase pathways could contribute to the induction and expansion of oxidative lesions characteristics of IBD and that apocynin could potentially be beneficial in IBD treatment.

American Psychological Association (APA)

Mouzaoui, Souad& Djerdjouri, Bahia& Makhezer, Nesrine& Kroviarski, Yolande& El Benna, Jamel& Dang, Pham My-Chan. 2014. Tumor Necrosis Factor-α-Induced Colitis Increases NADPH Oxidase 1 Expression, Oxidative Stress, and Neutrophil Recruitment in the Colon: Preventive Effect of Apocynin. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1043472

Modern Language Association (MLA)

Mouzaoui, Souad…[et al.]. Tumor Necrosis Factor-α-Induced Colitis Increases NADPH Oxidase 1 Expression, Oxidative Stress, and Neutrophil Recruitment in the Colon: Preventive Effect of Apocynin. Mediators of Inflammation No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1043472

American Medical Association (AMA)

Mouzaoui, Souad& Djerdjouri, Bahia& Makhezer, Nesrine& Kroviarski, Yolande& El Benna, Jamel& Dang, Pham My-Chan. Tumor Necrosis Factor-α-Induced Colitis Increases NADPH Oxidase 1 Expression, Oxidative Stress, and Neutrophil Recruitment in the Colon: Preventive Effect of Apocynin. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1043472

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1043472