MicroRNA-146a Decreases High GlucoseThrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression
Joint Authors
Peng, Ching-Tien
Wang, Huang-Joe
Huang, Yuan-Li
Shih, Ya-Yun
Wu, Hsing-Yu
Lo, Wan-Yu
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Diabetes is associated with hyperglycemia and increased thrombin production.
However, it is unknown whether a combination of high glucose and thrombin can modulate the expression of NAPDH oxidase (Nox) subtypes in human aortic endothelial cells (HAECs).
Moreover, we investigated the role of a diabetes-associated microRNA (miR-146a) in a diabetic atherothrombosis model.
We showed that high glucose (HG) exerted a synergistic effect with thrombin to induce a 10.69-fold increase in Nox4 mRNA level in HAECs.
Increased Nox4 mRNA expression was associated with increased Nox4 protein expression and ROS production.
Inflammatory cytokine kit identified that the treatment increased IL-8 and IL-6 levels.
Moreover, HG/thrombin treatment caused an 11.43-fold increase of THP-1 adhesion to HAECs.
In silico analysis identified the homology between miR-146a and the 3′-untranslated region of the Nox4 mRNA, and a luciferase reporter assay confirmed that the miR-146a mimic bound to this Nox4 regulatory region.
Additionally, miR-146a expression was decreased to 58% of that in the control, indicating impaired feedback restraint of HG/thrombin-induced endothelial inflammation.
In contrast, miR-146a mimic transfection attenuated HG/thrombin-induced upregulation of Nox4 expression, ROS generation, and inflammatory phenotypes.
In conclusion, miR-146a is involved in the regulation of endothelial inflammation via modulation of Nox4 expression in a diabetic atherothrombosis model.
American Psychological Association (APA)
Wang, Huang-Joe& Huang, Yuan-Li& Shih, Ya-Yun& Wu, Hsing-Yu& Peng, Ching-Tien& Lo, Wan-Yu. 2014. MicroRNA-146a Decreases High GlucoseThrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043514
Modern Language Association (MLA)
Wang, Huang-Joe…[et al.]. MicroRNA-146a Decreases High GlucoseThrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression. Mediators of Inflammation No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043514
American Medical Association (AMA)
Wang, Huang-Joe& Huang, Yuan-Li& Shih, Ya-Yun& Wu, Hsing-Yu& Peng, Ching-Tien& Lo, Wan-Yu. MicroRNA-146a Decreases High GlucoseThrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043514
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043514