Lancemaside A from Codonopsis lanceolata Modulates the Inflammatory Responses Mediated by Monocytes and Macrophages
Joint Authors
Yang, Woo Seok
Shin, Song Seok
Kim, Ji Hye
Kim, Han Gyung
Kim, Eunji
Rho, Ho Sik
Ko, Jaeyoung
Hong, Yong Deog
Cho, Jae Youl
Park, Jae Gwang
Sung, Gi-Ho
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-23
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In this study, we aimed to examine the cellular and molecular mechanisms of lancemaside A from Codonopsis lanceolata (Campanulaceae) in the inflammatory responses of monocytes (U937 cells) and macrophages (RAW264.7 cells).
Lancemaside A significantly suppressed the inflammatory functions of lipopolysaccharide- (LPS-) treated RAW264.7 cells by suppressing the production of nitric oxide (NO), the expression of the NO-producing enzyme inducible NO synthase (iNOS), the upregulation of the costimulatory molecule CD80, and the morphological changes induced by LPS exposure.
In addition, lancemaside A diminished the phagocytic activity of RAW264.7 cells and boosted the neutralizing capacity of these cells when treated with the radical generator sodium nitroprusside (SNP).
Interestingly, lancemaside A strongly blocked the adhesion activity of RAW264.7 cells to plastic culture plates, inhibited the cell-cell and cell-fibronectin (FN) adhesion of U937 cells that was triggered by treatment with an anti- β 1-integrin (CD29) antibody and immobilized FN, respectively.
By evaluating the activation of various intracellular signaling pathways and the levels of related nuclear transcription factors, lancemaside A was found to block the activation of inhibitor of κ B kinase (IKK) and p65/nuclear factor- (NF-) κ B.
Taken together, our findings strongly suggest that the anti-inflammatory function of lancemaside A is the result of its strong antioxidative and IKK/NF- κ B inhibitory activities.
American Psychological Association (APA)
Kim, Eunji& Yang, Woo Seok& Kim, Ji Hye& Park, Jae Gwang& Kim, Han Gyung& Ko, Jaeyoung…[et al.]. 2014. Lancemaside A from Codonopsis lanceolata Modulates the Inflammatory Responses Mediated by Monocytes and Macrophages. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043540
Modern Language Association (MLA)
Kim, Eunji…[et al.]. Lancemaside A from Codonopsis lanceolata Modulates the Inflammatory Responses Mediated by Monocytes and Macrophages. Mediators of Inflammation No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043540
American Medical Association (AMA)
Kim, Eunji& Yang, Woo Seok& Kim, Ji Hye& Park, Jae Gwang& Kim, Han Gyung& Ko, Jaeyoung…[et al.]. Lancemaside A from Codonopsis lanceolata Modulates the Inflammatory Responses Mediated by Monocytes and Macrophages. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043540
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043540