The Effects of Amphiregulin Induced MMP-13 Production in Human Osteoarthritis Synovial Fibroblast
Joint Authors
Chen, Yi-Te
Hou, Chun-Han
Hou, Sheng-Mou
Liu, Ju-Fang
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Osteoarthritis (OA) belongs to a group of degenerative diseases.
Synovial inflammation, cartilage abrasion, and subchondral sclerosis are characteristics of OA.
Researchers do not fully understand the exact etiology of OA.
However, matrix metalloproteinases (MMPs), which are responsible for cartilage matrix degradation, play a pivotal role in the progression of OA.
Amphiregulin (AREG) binds to the EGF receptor (EGFR) and activates downstream proteins.
AREG is involved in a variety of pathological processes, such as the development of tumors, inflammatory diseases, and rheumatoid arthritis.
However, the relationship between AREG and MMP-13 in OA synovial fibroblasts (SFs) remains unclear.
We investigated the signaling pathway involved in AREG-induced MMP-13 production in SFs.
AREG caused MMP-13 production in a concentration- and time-dependent manner.
The results of using pharmacological inhibitors and EGFR siRNA to block EGFR revealed that the EGFR receptor was involved in the AREG-mediated upregulation of MMP-13.
AREG-mediated MMP-13 production was attenuated by PI3K and Akt inhibitors.
The stimulation of cells by using AREG activated p65 phosphorylation and p65 translocation from the cytosol to the nucleus.
Our results provide evidence that AREG acts through the EGFR and activates PI3K, Akt, and finally NF-kappaB on the MMP-13 promoter, thus contributing to cartilage destruction during osteoarthritis.
American Psychological Association (APA)
Chen, Yi-Te& Hou, Chun-Han& Hou, Sheng-Mou& Liu, Ju-Fang. 2014. The Effects of Amphiregulin Induced MMP-13 Production in Human Osteoarthritis Synovial Fibroblast. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043797
Modern Language Association (MLA)
Chen, Yi-Te…[et al.]. The Effects of Amphiregulin Induced MMP-13 Production in Human Osteoarthritis Synovial Fibroblast. Mediators of Inflammation No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043797
American Medical Association (AMA)
Chen, Yi-Te& Hou, Chun-Han& Hou, Sheng-Mou& Liu, Ju-Fang. The Effects of Amphiregulin Induced MMP-13 Production in Human Osteoarthritis Synovial Fibroblast. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043797
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043797