اختبار كفاءة عزلات من البكتيريا Bacillus subtilis و الفطر Trichoderma hazianum في استحثاث بعض انزيمات الدفاعية ضد الفطر Alternaria solani في نبات الطماطمة

Abstract EN

The study was conducted in the plant protection greenhouse department’s during 2017-2018.

The study investigated two local isolates of Bacillus subtilis and Trichoderma hazianum to evaluate their efficacy as biocontrol agents in controlling early blight disease caused by Alternaria solani.

The results of the microscopic and biochemical tests showed that the isolates belonged to B.

subtilis.

The results indicated that these biocontrol agents could stimulate the resistance of systemic tomato plant by increasing the effectiveness of peroxidase, polyphenols oxidase and catalase enzymes in tomato leaves.

The highest increaseing in the efficacy of peroxidase was 1.286 and 1.358 (min / g wet weight) respectively when treated with Bacterial isolation II and T.

hazianum separately by three days before infection with A.

solani , while the increaseing was 1.263 (min / g wet weight) When treated with the Bacterial isolation compare infection of A.

solani after three days the enzyme efficacy in the control treatment, which amounted to 0.181.