New Insights into the Steen Solution Properties: Breakthrough in Antioxidant Effects via NOX2 Downregulation

Joint Authors

De Falco, Elena
Carnevale, Roberto
Pignatelli, Pasquale
Chimenti, Isotta
Venuta, Federico
Peruzzi, Mariangela
Marullo, Antonino G. M.
Frati, Giacomo
Biondi-Zoccai, Giuseppe
Anile, Marco
Diso, Daniele
Cavarretta, Elena
Sartini, Patrizio
Violi, Francesco

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-04-16

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Biology

Abstract EN

Ex vivo lung perfusion (EVLP) allows perfusion and reconditioning of retrieved lungs for organ transplantation.

The Steen solution is specifically designed for this procedure but the mechanism through which it elicits its activity is still to be fully clarified.

We speculated that Steen solution may encompass antioxidant properties allowing a reestablishment of pulmonary tissue homeostasis.

Blood samples from 10 healthy volunteers were recruited.

Platelets and white cells were incubated with Steen solution or buffer solution as control and stimulated with suitable agonists.

Reactive oxidant species (ROS), soluble NOX2 (sNOX2-derived peptide), a marker of NADPH oxidase activation, p 47 phox translocation to cell membrane and isoprostanes production, as marker of oxidative stress, and nitric oxide (NO), a powerful vasodilator and antioxidant molecule, were measured upon cell stimulation.

The Steen solution significantly inhibited p 47 phox translocation and NOX2 activation in platelets and white cells.

Consistent with this finding was the reduction of oxidative stress as documented by a significantly lowered formation of ROS and isoprostanes by both platelets and white cells.

Finally, cell incubation with Steen solution resulted in enhanced generation of NO.

Herewith, we provide the first evidence that Steen solution possesses antioxidant properties via downregulation of NADPH oxidase activity and enhanced production of NO.

American Psychological Association (APA)

Carnevale, Roberto& Biondi-Zoccai, Giuseppe& Peruzzi, Mariangela& De Falco, Elena& Chimenti, Isotta& Venuta, Federico…[et al.]. 2014. New Insights into the Steen Solution Properties: Breakthrough in Antioxidant Effects via NOX2 Downregulation. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1046979

Modern Language Association (MLA)

Carnevale, Roberto…[et al.]. New Insights into the Steen Solution Properties: Breakthrough in Antioxidant Effects via NOX2 Downregulation. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1046979

American Medical Association (AMA)

Carnevale, Roberto& Biondi-Zoccai, Giuseppe& Peruzzi, Mariangela& De Falco, Elena& Chimenti, Isotta& Venuta, Federico…[et al.]. New Insights into the Steen Solution Properties: Breakthrough in Antioxidant Effects via NOX2 Downregulation. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1046979

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1046979