DNA Methylation Is Involved in the Expression of miR-142-3p in Fibroblasts and Induced Pluripotent Stem Cells
Joint Authors
Abdul Razak, Siti Razila
Baba, Yukihiro
Nakauchi, Hiromitsu
Otsu, Makoto
Watanabe, Sumiko
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-02
Country of Publication
Egypt
No. of Pages
8
Abstract EN
MicroRNAs are differentially expressed in cells and regulate multiple biological processes.
We have been analyzing comprehensive expression patterns of microRNA in human and mouse embryonic stem and induced pluripotent stem cells.
We determined microRNAs specifically expressed in these pluripotent stem cells, and miR-142-3p is one of such microRNAs.
miR-142-3p is expressed at higher levels in induced pluripotent stem cells relative to fibroblasts in mice.
Level of expression of miR142-3p decreased during embryoid body formation from induced pluripotent stem cells.
Loss-of-function analyses of miR-142-3p suggested that miR-142-3p plays roles in the proliferation and differentiation of induced pluripotent stem cells.
CpG motifs were found in the 5′ genomic region of the miR-142-3p; they were highly methylated in fibroblasts, but not in undifferentiated induced pluripotent stem cells.
Treating fibroblasts with 5-aza-2′-deoxycytidine increased the expression of miR-142-3p significantly and reduced methylation at the CpG sites, suggesting that the expression of miR-142-3p is suppressed by DNA methylation in fibroblasts.
Luciferase analysis using various lengths of the 5′ genomic region of miR142-3p indicated that CpGs in the proximal enhancer region may play roles in suppressing the expression of miR-142-3p in fibroblasts.
American Psychological Association (APA)
Abdul Razak, Siti Razila& Baba, Yukihiro& Nakauchi, Hiromitsu& Otsu, Makoto& Watanabe, Sumiko. 2014. DNA Methylation Is Involved in the Expression of miR-142-3p in Fibroblasts and Induced Pluripotent Stem Cells. Stem Cells International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1047495
Modern Language Association (MLA)
Abdul Razak, Siti Razila…[et al.]. DNA Methylation Is Involved in the Expression of miR-142-3p in Fibroblasts and Induced Pluripotent Stem Cells. Stem Cells International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1047495
American Medical Association (AMA)
Abdul Razak, Siti Razila& Baba, Yukihiro& Nakauchi, Hiromitsu& Otsu, Makoto& Watanabe, Sumiko. DNA Methylation Is Involved in the Expression of miR-142-3p in Fibroblasts and Induced Pluripotent Stem Cells. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1047495
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047495