Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells
Joint Authors
Dai, Rui
Rossello, Ricardo
Chen, Chun-chun
Kessler, Joeran
Davison, Ian
Hochgeschwender, Ute
Jarvis, Erich D.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-11
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Pluripotent stem cells have the potential to become any cell in the adult body, including neurons and glia.
Avian stem cells could be used to study questions, like vocal learning, that would be difficult to examine with traditional mouse models.
Induced pluripotent stem cells (iPSCs) are differentiated cells that have been reprogrammed to a pluripotent stem cell state, usually using inducing genes or other molecules.
We recently succeeded in generating avian iPSC-like cells using mammalian genes, overcoming a limitation in the generation and use of iPSCs in nonmammalian species (Rosselló et al., 2013).
However, there were no established optimal cell culture conditions for avian iPSCs to establish long-term cell lines and thus to study neuronal differentiation in vitro.
Here we present an efficient method of maintaining chicken iPSC-like cells and for differentiating them into action potential generating neurons.
American Psychological Association (APA)
Dai, Rui& Rossello, Ricardo& Chen, Chun-chun& Kessler, Joeran& Davison, Ian& Hochgeschwender, Ute…[et al.]. 2015. Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells. Stem Cells International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1047498
Modern Language Association (MLA)
Dai, Rui…[et al.]. Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells. Stem Cells International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1047498
American Medical Association (AMA)
Dai, Rui& Rossello, Ricardo& Chen, Chun-chun& Kessler, Joeran& Davison, Ian& Hochgeschwender, Ute…[et al.]. Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells. Stem Cells International. 2015. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1047498
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047498