Transient Downregulation of Nanog and Oct4 Induced by DETANO Exposure in Mouse Embryonic Stem Cells Leads to MesodermalEndodermal Lineage Differentiation
Joint Authors
Mora-Castilla, Sergio
Tejedo, Juan R.
Tapia-Limonchi, Rafael
Díaz, Irene
Hitos, Ana B.
Cahuana, Gladys M.
Hmadcha, Abdelkrim
Martín, Franz
Soria, Bernat
Bedoya, Francisco J.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-03
Country of Publication
Egypt
No. of Pages
11
Abstract EN
The function of pluripotency genes in differentiation is a matter of investigation.
We report here that Nanog and Oct4 are reexpressed in two mouse embryonic stem cell (mESC) lines following exposure to the differentiating agent DETA/NO.
Both cell lines express a battery of both endoderm and mesoderm markers following induction of differentiation with DETA/NO-based protocols.
Confocal analysis of cells undergoing directed differentiation shows that the majority of cells expressing Nanog express also endoderm genes such as Gata4 and FoxA2 (75.4% and 96.2%, resp.).
Simultaneously, mRNA of mesodermal markers Flk1 and Mef2c are also regulated by the treatment.
Acetylated histone H3 occupancy at the promoter of Nanog is involved in the process of reexpression.
Furthermore, Nanog binding to the promoter of Brachyury leads to repression of this gene, thus disrupting mesendoderm transition.
American Psychological Association (APA)
Mora-Castilla, Sergio& Tejedo, Juan R.& Tapia-Limonchi, Rafael& Díaz, Irene& Hitos, Ana B.& Cahuana, Gladys M.…[et al.]. 2014. Transient Downregulation of Nanog and Oct4 Induced by DETANO Exposure in Mouse Embryonic Stem Cells Leads to MesodermalEndodermal Lineage Differentiation. Stem Cells International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047509
Modern Language Association (MLA)
Mora-Castilla, Sergio…[et al.]. Transient Downregulation of Nanog and Oct4 Induced by DETANO Exposure in Mouse Embryonic Stem Cells Leads to MesodermalEndodermal Lineage Differentiation. Stem Cells International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1047509
American Medical Association (AMA)
Mora-Castilla, Sergio& Tejedo, Juan R.& Tapia-Limonchi, Rafael& Díaz, Irene& Hitos, Ana B.& Cahuana, Gladys M.…[et al.]. Transient Downregulation of Nanog and Oct4 Induced by DETANO Exposure in Mouse Embryonic Stem Cells Leads to MesodermalEndodermal Lineage Differentiation. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047509
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047509