The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells
Joint Authors
Pires, Ana O.
Neves-Carvalho, Andreia
Sousa, Nuno
Salgado, Antonio
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-15
Country of Publication
Egypt
No. of Pages
10
Abstract EN
The goal of this study was to determine and compare the effects of the secretome of mesenchymal stem cells (MSCs) isolated from human bone-marrow (BMSCs) and the Wharton jelly surrounding the vein and arteries of the umbilical cord (human umbilical cord perivascular cells (HUCPVCs)) on the survival and differentiation of a human neuroblastoma cell line (SH-SY5Y).
For this purpose, SH-SY5Y cells were differentiated with conditioned media (CM) from the MSCs populations referred above.
Retinoic acid cultured cells were used as control for neuronal differentiated SH-SY5Y cells.
SH-SY5Y cells viability assessment revealed that the secretome of BMSCs and HUCPVCs, in the form of CM, was able to induce their survival.
Moreover, immunocytochemical experiments showed that CM from both MSCs was capable of inducing neuronal differentiation of SH-SY5Y cells.
Finally, neurite lengths assessment and quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) analysis demonstrated that CM from BMSCs and HUCPVCs differently induced neurite outgrowth and mRNA levels of neuronal markers exhibited by SH-SY5Y cells.
Overall, our results show that the secretome of both BMSCs and HUCPVCs was capable of supporting SH-SY5Y cells survival and promoting their differentiation towards a neuronal phenotype.
American Psychological Association (APA)
Pires, Ana O.& Neves-Carvalho, Andreia& Sousa, Nuno& Salgado, Antonio. 2014. The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells. Stem Cells International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047518
Modern Language Association (MLA)
Pires, Ana O.…[et al.]. The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells. Stem Cells International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1047518
American Medical Association (AMA)
Pires, Ana O.& Neves-Carvalho, Andreia& Sousa, Nuno& Salgado, Antonio. The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047518
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047518