Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells
Joint Authors
Mohtasebi, Maryam Sadat
Nasri, Fatemeh
Kamali Sarvestani, Eskandar
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-11
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Mesenchymal stem cells (MSCs) have been used to treat a variety of degenerative disorders.
Labeling of MSCs with an appropriate tracer is vital to demonstrate the in vivo engraftment and differentiation of transplanted MSCs.
DiD is a lipophilic fluorescent dye with near infrared emission spectra that makes it suitable for in vivo tracing.
Therefore, in the present study the consequences of DiD labeling on induction of oxidative stress and apoptosis as well as inhibition of biological functions of mesenchymal stem cells (MSCs) were investigated.
DiD labeling did not provoke the production of ROS, induction of apoptosis, or inhibition of production of immunosuppressive factors (PGE2 and IL-10) by MSCs.
In addition, there were no statistical differences between DiD-labeled and unlabeled MSCs in suppression of proliferation and cytokine production (IFN- γ and IL-17) by in vitro stimulated splenocytes or improvement of clinical score in EAE after in vivo administration.
In addition, DiD labeling did not alter the differentiation capacity of MSCs.
Taken together, DiD can be considered as a safe dye for in vivo tracking of MSCs.
American Psychological Association (APA)
Mohtasebi, Maryam Sadat& Nasri, Fatemeh& Kamali Sarvestani, Eskandar. 2014. Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells. Stem Cells International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047520
Modern Language Association (MLA)
Mohtasebi, Maryam Sadat…[et al.]. Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells. Stem Cells International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1047520
American Medical Association (AMA)
Mohtasebi, Maryam Sadat& Nasri, Fatemeh& Kamali Sarvestani, Eskandar. Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047520
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047520