Cell Therapy for Chemically Induced Ovarian Failure in Mice
Joint Authors
Terraciano, Paula
Garcez, Tuane
Ayres, Laura
Durli, Isabel
Baggio, Melchiani
Kuhl, Cristiana Palma
Laurino, Claudia
Passos, Eduardo
Paz, Ana Helena
Cirne-Lima, Elizabeth
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-04
Country of Publication
Egypt
No. of Pages
8
Abstract EN
Cell therapy has been linked to an unexplained return of ovarian function and fertility in some cancer survivors.
Studies modeling this in mice have shown that cells transplantation generates donor-derived oocytes in chemotherapy-treated recipients.
This study was conducted to further clarify the impact of cell transplantation from different sources on female reproductive function after chemotherapy using a preclinical mouse model.
Methods.
Female mice were administered 7.5 mg/kg cisplatin followed by cell transplantation (one week later) using GFP+ female cell donors.
For cell tracking, adipose derived stem cell GFP+ (ADSC), female germline stem cell GFP+/MVH+ (FGSC), or ovary cell suspension GFP+ mice were transplanted into cisplatin-treated wild-type recipients.
After 7 or 14 days animals were killed and histological analysis, IHQ for GFP cells, and ELISA for estradiol were performed.
Results.
Histological examinations showed that ADSC, ovary cell suspension, and FGSC transplant increase the number of follicles with apparent normal structure in the cells recipient group euthanized on day 7.
Cell tracking showed GFP+ samples 7 days after transplant.
Conclusion.
These data suggest that intraovarian injection of ADSCs and FGSC into mice with chemotherapy-induced ovarian failure diminished the damage caused by cisplatin.
American Psychological Association (APA)
Terraciano, Paula& Garcez, Tuane& Ayres, Laura& Durli, Isabel& Baggio, Melchiani& Kuhl, Cristiana Palma…[et al.]. 2014. Cell Therapy for Chemically Induced Ovarian Failure in Mice. Stem Cells International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1047529
Modern Language Association (MLA)
Terraciano, Paula…[et al.]. Cell Therapy for Chemically Induced Ovarian Failure in Mice. Stem Cells International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1047529
American Medical Association (AMA)
Terraciano, Paula& Garcez, Tuane& Ayres, Laura& Durli, Isabel& Baggio, Melchiani& Kuhl, Cristiana Palma…[et al.]. Cell Therapy for Chemically Induced Ovarian Failure in Mice. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1047529
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047529