A Comparative View on Human Somatic Cell Sources for iPSC Generation
Joint Authors
Linta, Leonhard
Raab, Stefanie
Klingenstein, Moritz
Liebau, Stefan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-06
Country of Publication
Egypt
No. of Pages
12
Abstract EN
The breakthrough of reprogramming human somatic cells was achieved in 2006 by the work of Yamanaka and Takahashi.
From this point, fibroblasts are the most commonly used primary somatic cell type for the generation of induced pluripotent stem cells (iPSCs).
Various characteristics of fibroblasts supported their utilization for the groundbreaking experiments of iPSC generation.
One major advantage is the high availability of fibroblasts which can be easily isolated from skin biopsies.
Furthermore, their cultivation, propagation, and cryoconservation properties are uncomplicated with respect to nutritional requirements and viability in culture.
However, the required skin biopsy remains an invasive approach, representing a major drawback for using fibroblasts as the starting material.
More and more studies appeared over the last years, describing the reprogramming of other human somatic cell types.
Cells isolated from blood samples or urine, as well as more unexpected cell types, like pancreatic islet beta cells, synovial cells, or mesenchymal stromal cells from wisdom teeth, show promising characteristics for a reprogramming strategy.
Here, we want to highlight the advantages of keratinocytes from human plucked hair as a widely usable, noninvasive harvesting method for primary material in comparison with other commonly used cell types.
American Psychological Association (APA)
Raab, Stefanie& Klingenstein, Moritz& Liebau, Stefan& Linta, Leonhard. 2014. A Comparative View on Human Somatic Cell Sources for iPSC Generation. Stem Cells International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047531
Modern Language Association (MLA)
Raab, Stefanie…[et al.]. A Comparative View on Human Somatic Cell Sources for iPSC Generation. Stem Cells International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1047531
American Medical Association (AMA)
Raab, Stefanie& Klingenstein, Moritz& Liebau, Stefan& Linta, Leonhard. A Comparative View on Human Somatic Cell Sources for iPSC Generation. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047531
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047531