Conjugates of a Photoactivated Rhodamine with Biopolymers for Cell Staining
Joint Authors
Zaitsev, Sergei Yu.
Shaposhnikov, Mikhail N.
Solovyeva, Daria O.
Solovyeva, Valeria V.
Rizvanov, Albert A.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Conjugates of the photoactivated rhodamine dyes with biopolymers (proteins, polysaccharides, and nucleic acids) are important tools for microscopic investigation of biological tissue.
In this study, a precursor of the photoactivated fluorescent dye (PFD) has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan (“Chitosan-PFD”) and histone H1 (“Histone H1.3-PFD”).
The intensive fluorescence has been observed after photoactivation of these conjugates inside cells (A431, HaCaT, HEK239, HBL-100, and MDCK).
Developed procedures and obtained data are important for further application of novel precursors of fluorescent dyes (“caged” dyes) for microscopic probing of biological objects.
Thus, the synthesized “Chitosan-PFD” and “Histone H1-PFD” have been successfully applied in this study for intracellular transport visualization by fluorescent microscopy.
American Psychological Association (APA)
Zaitsev, Sergei Yu.& Shaposhnikov, Mikhail N.& Solovyeva, Daria O.& Solovyeva, Valeria V.& Rizvanov, Albert A.. 2014. Conjugates of a Photoactivated Rhodamine with Biopolymers for Cell Staining. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1049076
Modern Language Association (MLA)
Zaitsev, Sergei Yu.…[et al.]. Conjugates of a Photoactivated Rhodamine with Biopolymers for Cell Staining. The Scientific World Journal No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1049076
American Medical Association (AMA)
Zaitsev, Sergei Yu.& Shaposhnikov, Mikhail N.& Solovyeva, Daria O.& Solovyeva, Valeria V.& Rizvanov, Albert A.. Conjugates of a Photoactivated Rhodamine with Biopolymers for Cell Staining. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1049076
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049076