NMR Spectroscopy of Human Eye Tissues: A New Insight into Ocular Biochemistry
Joint Authors
Kryczka, Tomasz
Wylęgała, Edward
Dobrowolski, Dariusz
Midelfart, Anna
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Background.
The human eye is a complex organ whose anatomy and functions has been described very well to date.
Unfortunately, the knowledge of the biochemistry and metabolic properties of eye tissues varies.
Our objective was to reveal the biochemical differences between main tissue components of human eyes.
Methods.
Corneas, irises, ciliary bodies, lenses, and retinas were obtained from cadaver globes 0-1/2 hours postmortem of 6 male donors (age: 44–61 years).
The metabolic profile of tissues was investigated with HR MAS 1H NMR spectroscopy.
Results.
A total of 29 metabolites were assigned in the NMR spectra of the eye tissues.
Significant differences between tissues were revealed in contents of the most distant eye-tissues, while irises and ciliary bodies showed minimal biochemical differences.
ATP, acetate, choline, glutamate, lactate, myoinositol, and taurine were identified as the primary biochemical compounds responsible for differentiation of the eye tissues.
Conclusions.
In this study we showed for the first time the results of the analysis of the main human eye tissues with NMR spectroscopy.
The biochemical contents of the selected tissues seemed to correspond to their primary anatomical and functional attributes, the way of the delivery of the nutrients, and the location of the tissues in the eye.
American Psychological Association (APA)
Kryczka, Tomasz& Wylęgała, Edward& Dobrowolski, Dariusz& Midelfart, Anna. 2014. NMR Spectroscopy of Human Eye Tissues: A New Insight into Ocular Biochemistry. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1050074
Modern Language Association (MLA)
Kryczka, Tomasz…[et al.]. NMR Spectroscopy of Human Eye Tissues: A New Insight into Ocular Biochemistry. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1050074
American Medical Association (AMA)
Kryczka, Tomasz& Wylęgała, Edward& Dobrowolski, Dariusz& Midelfart, Anna. NMR Spectroscopy of Human Eye Tissues: A New Insight into Ocular Biochemistry. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1050074
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050074