Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
Joint Authors
Fang, Jie
Han, Xiaojiao
Xie, Lihua
Liu, Mingying
Qiao, Guirong
Jiang, Jing
Zhuo, Renying
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The halotolerant fungus Aspergillus glaucus CCHA was isolated from the surface of wild vegetation around a saltern with the salinity range being 0–31%.
Here, a full-length cDNA library of A.
glaucus under salt stress was constructed to identify genes related to salt tolerance, and one hundred clones were randomly selected for sequencing and bioinformatics analysis.
Among these, 82 putative sequences were functionally annotated as being involved in signal transduction, osmolyte synthesis and transport, or regulation of transcription.
Subsequently, the cDNA library was transformed into E.
coli cells to screen for putative salt stress-related clones.
Five putative positive clones were obtained from E.
coli cells grown on LB agar containing 1 M NaCl, on which they showed rapid growth compared to the empty vector control line.
Analysis of transgenic Arabidopsis thaliana lines overexpressing CCHA-2142 demonstrated that the gene conferred increased salt tolerance to plants as well by protecting the cellular membranes, suppressing the inhibition of chlorophyll biosynthesis.
These results highlight the utility of this A.
glaucus cDNA library as a tool for isolating and characterizing genes related to salt tolerance.
Furthermore, the identified genes can be used for the study of the underlying biology of halotolerance.
American Psychological Association (APA)
Fang, Jie& Han, Xiaojiao& Xie, Lihua& Liu, Mingying& Qiao, Guirong& Jiang, Jing…[et al.]. 2014. Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1050380
Modern Language Association (MLA)
Fang, Jie…[et al.]. Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli. The Scientific World Journal No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1050380
American Medical Association (AMA)
Fang, Jie& Han, Xiaojiao& Xie, Lihua& Liu, Mingying& Qiao, Guirong& Jiang, Jing…[et al.]. Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1050380
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050380