Enzymatic Activity Analysis and Catalytic Essential Residues Identification of Brucella abortus Malate Dehydrogenase
Joint Authors
Yu, Shengqing
Ding, Chan
Han, Xiangan
Tong, Yongliang
Tian, Mingxing
Zhang, Yuxi
Sun, Xiaoqing
Wang, Shaohui
Qiu, Xusheng
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Malate dehydrogenase (MDH) plays important metabolic roles in bacteria.
In this study, the recombinant MDH protein (His-MDH) of Brucella abortus was purified and its ability to catalyze the conversion of oxaloacetate (OAA) to L-malate (hereon referred to as MDH activity) was analyzed.
Michaelis Constant (Km) and Maximum Reaction Velocity (Vmax) of the reaction were determined to be 6.45×10−3 M and 0.87 mM L−1 min−1, respectively.
In vitro studies showed that His-MDH exhibited maximal MDH activity in pH 6.0 reaction buffer at 40°C.
The enzymatic activity was 100%, 60%, and 40% inhibited by Cu2+, Zn2+, and Pb2+, respectively.
In addition, six amino acids in the MDH were mutated to investigate their roles in the enzymatic activity.
The results showed that the substitutions of amino acids Arg 89, Asp 149, Arg 152, His 176, or Thr 231 almost abolished the activity of His-MDH.
The present study will help to understand MDH’s roles in B.
abortus metabolism.
American Psychological Association (APA)
Han, Xiangan& Tong, Yongliang& Tian, Mingxing& Zhang, Yuxi& Sun, Xiaoqing& Wang, Shaohui…[et al.]. 2014. Enzymatic Activity Analysis and Catalytic Essential Residues Identification of Brucella abortus Malate Dehydrogenase. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1051823
Modern Language Association (MLA)
Han, Xiangan…[et al.]. Enzymatic Activity Analysis and Catalytic Essential Residues Identification of Brucella abortus Malate Dehydrogenase. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1051823
American Medical Association (AMA)
Han, Xiangan& Tong, Yongliang& Tian, Mingxing& Zhang, Yuxi& Sun, Xiaoqing& Wang, Shaohui…[et al.]. Enzymatic Activity Analysis and Catalytic Essential Residues Identification of Brucella abortus Malate Dehydrogenase. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1051823
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051823