Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines
Joint Authors
Di Bernardo, Giovanni
Castello, Giuseppe
Colonna, Giovanni
Costantini, Susan
Guariniello, Stefano
Cammarota, Marcella
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is still one of the most fatal cancers.
Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis.
Since the selenium is able to fight the oxidative damage which is one of the major origins of cell damage as well as cancer, we have recently focused our attention on selenoprotein family and their involvement in HCC.
In the present paper we have carried out a global analysis of the selenotranscriptome expression in HepG2 and Huh7 cells compared to the normal human hepatocytes by reverse transcription-qPCR (RT-qPCR).
Our data showed that in both cells there are three downregulated (DIO1, DIO2, and SELO) and ten upregulated (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1) genes.
Additionally, interactomic studies were carried out to evaluate the ability of these down- and upregulated genes to interact between them as well as to identify putative HUB nodes representing the centers of correlation able to exercise a direct control over the coordinated genes.
American Psychological Association (APA)
Guariniello, Stefano& Di Bernardo, Giovanni& Colonna, Giovanni& Cammarota, Marcella& Castello, Giuseppe& Costantini, Susan. 2015. Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines. Analytical Cellular Pathology،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1052354
Modern Language Association (MLA)
Guariniello, Stefano…[et al.]. Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines. Analytical Cellular Pathology No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1052354
American Medical Association (AMA)
Guariniello, Stefano& Di Bernardo, Giovanni& Colonna, Giovanni& Cammarota, Marcella& Castello, Giuseppe& Costantini, Susan. Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines. Analytical Cellular Pathology. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1052354
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1052354