Melatonin Modulates Endoplasmic Reticulum Stress and AktGSK3-Beta Signaling Pathway in a Rat Model of Renal Warm Ischemia Reperfusion
Joint Authors
Hadj Ayed Tka, Kaouther
Mahfoudh Boussaid, Asma
Zaouali, Mohamed Amine
Kammoun, Rym
Bejaoui, Mohamed
Ghoul Mazgar, Sonia
Rosello Catafau, Joan
Ben Abdennebi, Hassen
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Melatonin (Mel) is widely used to attenuate ischemia/reperfusion (I/R) injury in several organs.
Nevertheless, the underlying mechanisms remain unclear.
This study was conducted to explore the effect of Mel on endoplasmic reticulum (ER) stress, Akt and MAPK cascades after renal warm I/R.
Eighteen Wistar rats were randomized into three groups: Sham, I/R, and Mel + I/R.
The ischemia period was 60 min followed by 120 min of reperfusion.
Mel (10 mg/kg) was administrated 30 min prior to ischemia.
The creatinine clearance, MDA, LDH levels, and histopathological changes were evaluated.
In addition, Western blot was performed to study ER stress and its downstream apoptosis as well as phosphorylation of Akt, GSK-3β, VDAC, ERK, and P38.
Mel decreased cytolysis and lipid peroxidation and improved renal function and morphology compared to I/R group.
Parallely, it significantly reduced the ER stress parameters including GRP 78, p-PERK, XBP 1, ATF 6, CHOP, and JNK.
Simultaneously, p-Akt level was significantly enhanced and its target molecules GSK-3β and VDAC were inhibited.
Furthermore, the ERK and P38 phosphorylation were evidently augmented after Mel administration in comparison to I/R group.
In conclusion, Mel improves the recovery of renal function by decreasing ER stress and stimulating Akt pathway after renal I/R injury.
American Psychological Association (APA)
Hadj Ayed Tka, Kaouther& Mahfoudh Boussaid, Asma& Zaouali, Mohamed Amine& Kammoun, Rym& Bejaoui, Mohamed& Ghoul Mazgar, Sonia…[et al.]. 2015. Melatonin Modulates Endoplasmic Reticulum Stress and AktGSK3-Beta Signaling Pathway in a Rat Model of Renal Warm Ischemia Reperfusion. Analytical Cellular Pathology،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1052363
Modern Language Association (MLA)
Hadj Ayed Tka, Kaouther…[et al.]. Melatonin Modulates Endoplasmic Reticulum Stress and AktGSK3-Beta Signaling Pathway in a Rat Model of Renal Warm Ischemia Reperfusion. Analytical Cellular Pathology No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1052363
American Medical Association (AMA)
Hadj Ayed Tka, Kaouther& Mahfoudh Boussaid, Asma& Zaouali, Mohamed Amine& Kammoun, Rym& Bejaoui, Mohamed& Ghoul Mazgar, Sonia…[et al.]. Melatonin Modulates Endoplasmic Reticulum Stress and AktGSK3-Beta Signaling Pathway in a Rat Model of Renal Warm Ischemia Reperfusion. Analytical Cellular Pathology. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1052363
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1052363