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Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus
Joint Authors
Mahanta, Jagadish
Khan, Siraj A.
Topno, Rashmee
Chowdhury, Pritom
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-27
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
West Nile Virus (WNV) is a pathogenic arbovirus that belongs to genus Flavivirus under family Flaviviridae.
Till now there are no approved vaccines against WNV for human use.
In this study, the effect of two alkylating agents, formaldehyde and β-PL, generally used for inactivated vaccine preparation, was assessed on the basis of antigenic and immunogenic potential of the inactivated WNV.
Lineage 5 WNV isolates were inactivated by both formalin and β-PL treatments.
Inactivation was confirmed by repeated passage in BHK-21 cell line and infant mice.
Viruses inactivated by both the treatments showed higher antigenicity.
Immune response in mice model showed serum anti-WNV antibody titre was moderately higher in formalin inactivated antigen compared to β-PL inactivated antigen.
However, no significant differences were observed in neutralization antibody titre.
In conclusion, we can state that both formaldehyde and β-PL inactivation processes were found to be equally efficient for inactivation of WNV.
However, they need to be compared with other inactivating agents along with study on cell mediated immune response.
American Psychological Association (APA)
Chowdhury, Pritom& Topno, Rashmee& Khan, Siraj A.& Mahanta, Jagadish. 2015. Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus. Advances in Virology،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1054029
Modern Language Association (MLA)
Chowdhury, Pritom…[et al.]. Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus. Advances in Virology No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1054029
American Medical Association (AMA)
Chowdhury, Pritom& Topno, Rashmee& Khan, Siraj A.& Mahanta, Jagadish. Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus. Advances in Virology. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1054029
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054029