β-Elemene Inhibits Cell Proliferation by Regulating the Expression and Activity of Topoisomerases I and IIα in Human Hepatocarcinoma HepG-2 Cells
Joint Authors
Gong, Min
Liu, Ying
Zhang, Jian
Gao, Ya-jie
Zhai, Ping-ping
Su, Xi
Li, Xiang
Li, Yan
Hou, Li
Cui, Xiao-nan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
To investigate the effects of β-Elemene (β-ELE) on the proliferation, apoptosis, and topoisomerase I (TOPO I) and topoisomerase IIα (TOPO IIα) expression and activity of human hepatocarcinoma HepG-2 cells.
Methods.
After treatment with β-ELE, morphological alterations of HepG-2 cells were observed under an inverted microscope.
Cell proliferation was assessed using an MTT assay, cell cycles were analyzed using flow cytometry, and apoptosis was detected by Annexin V/PI staining.
The expression of TOPO I and TOPO IIα was analyzed by Western blot techniques, and their activity was measured using the TOPO I-mediated, supercoiled pBR322 DNA relaxation and TOPO IIα-mediated Kinetoplast DNA (kDNA) decatenation assays, respectively.
Supercoiled pBR322 and kDNA were also used to determine the direct effect of β-ELE on DNA breaks.
Results.
β-ELE significantly inhibited HepG-2 cell proliferation in a dose- and time-dependent manner.
β-ELE also induced tumor cell arrest at S phase, induced cell apoptosis, and downregulated the protein expression of TOPO I and TOPO IIα in a dose-dependent manner.
β-ELE also inhibited TOPO I- and TOPO IIα-mediated DNA relaxation but did not directly induce DNA breakage at any concentration.
Conclusion.
β-ELE could inhibit the proliferation of HepG-2 cells and interfere with the expression and activity of TOPO I and TOPO IIα.
American Psychological Association (APA)
Gong, Min& Liu, Ying& Zhang, Jian& Gao, Ya-jie& Zhai, Ping-ping& Su, Xi…[et al.]. 2015. β-Elemene Inhibits Cell Proliferation by Regulating the Expression and Activity of Topoisomerases I and IIα in Human Hepatocarcinoma HepG-2 Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054395
Modern Language Association (MLA)
Gong, Min…[et al.]. β-Elemene Inhibits Cell Proliferation by Regulating the Expression and Activity of Topoisomerases I and IIα in Human Hepatocarcinoma HepG-2 Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1054395
American Medical Association (AMA)
Gong, Min& Liu, Ying& Zhang, Jian& Gao, Ya-jie& Zhai, Ping-ping& Su, Xi…[et al.]. β-Elemene Inhibits Cell Proliferation by Regulating the Expression and Activity of Topoisomerases I and IIα in Human Hepatocarcinoma HepG-2 Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054395
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054395