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Missense Mutations in Exons 18–24 of EGFR in Hepatocellular Carcinoma Tissues
Joint Authors
Panvichian, Ravat
Tantiwetrueangdet, Anchalee
Leelaudomlipi, Surasak
Sornmayura, Pattana
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Epidermal growth factor receptor (EGFR), a transmembrane tyrosine kinase receptor, plays important roles in various cancers.
In nonsmall cell lung cancer (NSCLC), EGFR mutations cluster around the ATP-binding pocket (exons 18–21) and some of these mutations activate the kinase and induce an increased sensitivity to EGFR-tyrosine kinase inhibitors.
Nevertheless, data of EGFR mutations in HCC are limited.
In this study, we investigated EGFR expression by immunohistochemistry and EGFR mutations (exons 18–24) by PCR cloning and sequencing.
EGFR overexpression in HCC and matched nontumor tissues were detected in 13/40 (32.5%) and 10/35 (28.6%), respectively.
Moreover, missense and silent mutations were detected in 13/33 (39.4%) and 11/33 (33.3%) of HCC tissues, respectively.
The thirteen different missense mutations were p.L730P, p.V742I, p.K757E, p.I780T, p.N808S, p.R831C, p.V851A, p.V897A, p.S912P, p.P937L, p.T940A, p.M947V, and p.M947T.
We also found already known SNP, p.Q787Q (CAG>CAA), in 13/33 (39.4%) of HCC tissues.
However, no significant association was detected between EGFR mutations and EGFR overexpression, tissue, age, sex, tumor size, AFP, HBsAg, TP53, and Ki-67.
Further investigation is warranted to validate the frequency and activity of these missense mutations, as well as their roles in HCC tumorigenesis and in EGFR-targeted therapy.
American Psychological Association (APA)
Panvichian, Ravat& Tantiwetrueangdet, Anchalee& Sornmayura, Pattana& Leelaudomlipi, Surasak. 2015. Missense Mutations in Exons 18–24 of EGFR in Hepatocellular Carcinoma Tissues. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1054483
Modern Language Association (MLA)
Panvichian, Ravat…[et al.]. Missense Mutations in Exons 18–24 of EGFR in Hepatocellular Carcinoma Tissues. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1054483
American Medical Association (AMA)
Panvichian, Ravat& Tantiwetrueangdet, Anchalee& Sornmayura, Pattana& Leelaudomlipi, Surasak. Missense Mutations in Exons 18–24 of EGFR in Hepatocellular Carcinoma Tissues. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1054483
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054483