Purification and Characterization of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus penicillioides Isolate and Its Potential for Souse with Detergents
Joint Authors
Ali, Imran
Anwar, Mohammad
Prasongsuk, Sehanat
Lotrakul, Pongtharin
Punnapayak, Hunsa
Akbar, Ali
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
An extracellular α-amylase from the obligate halophilic Aspergillus penicillioides TISTR3639 strain was produced and enriched to apparent homogeneity by ammonium sulfate precipitation and Sephadex G100 gel filtration column chromatography.
The mass of the purified amylase was estimated to be 42 kDa by SDS-PAGE.
With soluble starch as the substrate it had a specific activity of 118.42 U·mg−1 and Vmax and Km values of 1.05 µmol·min−1·mg−1 and 5.41 mg·mL−1, respectively.
The enzyme was found to have certain polyextremophilic characteristics, with an optimum activity at pH 9, 80°C, and 300 g·L−1 NaCl.
The addition of CaCl2 at 2 mM was found to slightly enhance the amylase activity, while ZnCl2, FeCl2, or EDTA at 2 mM was strongly or moderately inhibitory, respectively, suggesting the requirement for a (non-Fe2+ or Zn2+) divalent cation.
The enzyme retained more than 80% of its activity when incubated with three different laundry detergents and had a better performance compared to a commercial amylase and three detergents in the presence of increasing NaCl concentrations up to 300 g·L−1.
Accordingly, it has a good potential for use as an α-amylase in a low water activity (high salt concentration) and at high pH and temperatures.
American Psychological Association (APA)
Ali, Imran& Akbar, Ali& Anwar, Mohammad& Prasongsuk, Sehanat& Lotrakul, Pongtharin& Punnapayak, Hunsa. 2015. Purification and Characterization of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus penicillioides Isolate and Its Potential for Souse with Detergents. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054737
Modern Language Association (MLA)
Ali, Imran…[et al.]. Purification and Characterization of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus penicillioides Isolate and Its Potential for Souse with Detergents. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054737
American Medical Association (AMA)
Ali, Imran& Akbar, Ali& Anwar, Mohammad& Prasongsuk, Sehanat& Lotrakul, Pongtharin& Punnapayak, Hunsa. Purification and Characterization of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus penicillioides Isolate and Its Potential for Souse with Detergents. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054737