Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients
Joint Authors
Wylęgała, Edward
Dobrowolski, Dariusz
Aragona, Pasquale
Puzzolo, Domenico
Micali, Antonio
Orzechowska-Wylegala, Boguslawa
Wowra, Bogumil
Wroblewska-Czajka, Ewa
Grolik, Maria
Szczubialka, Krzysztof
Nowakowska, Maria
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-16
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients.
Methods.
Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium.
For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment.
Suspension of single cells was seeded on previously prepared denuded amniotic membrane.
Cultures were carried on culture dishes inserts in the presence of the inactivated with Mitomycin C monolayer of 3T3 fibroblasts.
Cultures were carried for seven days.
Stratified oral mucosa epithelium with its amniotic membrane carrier was transplanted on the surgically denuded corneal surface of aniridia patients with total or subtotal limbal stem cell deficiency.
Outcome Measures.
Corneal surface, epithelial regularity, and visual acuity improvement were evaluated.
Results.
At the end of the observation period, 76.4% of the eyes had regular transparent epithelium and 23.5% had developed epithelial defects or central corneal haze; in 88.2% of cases visual acuity had increased.
VA range was from HM 0.05 before the surgery to HM up to 0.1 after surgery.
Conclusion.
Application of cultivated oral mucosa epithelium restores regular epithelium on the corneal surface with moderate improvement in quality of vision.
American Psychological Association (APA)
Dobrowolski, Dariusz& Orzechowska-Wylegala, Boguslawa& Wowra, Bogumil& Wroblewska-Czajka, Ewa& Grolik, Maria& Szczubialka, Krzysztof…[et al.]. 2015. Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1054867
Modern Language Association (MLA)
Dobrowolski, Dariusz…[et al.]. Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1054867
American Medical Association (AMA)
Dobrowolski, Dariusz& Orzechowska-Wylegala, Boguslawa& Wowra, Bogumil& Wroblewska-Czajka, Ewa& Grolik, Maria& Szczubialka, Krzysztof…[et al.]. Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1054867
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054867