Effects of a Particular Heptapeptide on the IFN-α-Sensitive CML Cells
Joint Authors
Wan, Xin-xing
Chen, Han-chun
Yang, Fu-lan
Zhang, Dian-zheng
Chen, Fang-zhi
Zhou, Xi
Zhou, Mei-juan
Fu, Jun-jiang
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Using the phage display biopanning technique, we have previously identified a heptapeptide KLWVIPQ which specifically binds to the surface of the IFN-α-sensitive but not the IFN-α-resistant CML cells.
The effects of this heptapeptide on the IFN-α-sensitive CML cells were investigated in the present study.
IFN-α-sensitive KT-1/A3 and IFN-α-resistant KT-1/A3R CML cells were transfected by pEGFP-KLWVIPQ expression vector and/or induced by IFN-α.
WST-1 cell proliferation assay, flow cytometry, and western blotting were performed to determine the effects of this heptapeptide and/or IFN-α on CML cells.
The viability of the KT-1/A3 cells was inhibited and apoptosis was induced by either expression of the heptapeptide KLWVIPQ or IFN-α treatment with concurrent upregulation of P53 and downregulation of P210bcr/abl.
However, these effects were not observed in the IFN-α-resistant KT-1/A3R cells.
These results suggest that the heptapeptide KLWVIPQ shares a similar mechanism with IFN-α in the regulation of CML cell growth and apoptosis, implying that the heptapeptide KLWVIPQ could be a novel target to go further into mechanisms of IFN-α sensitivity and/or resistance in CML.
American Psychological Association (APA)
Yang, Fu-lan& Chen, Fang-zhi& Wan, Xin-xing& Zhou, Xi& Zhou, Mei-juan& Chen, Han-chun…[et al.]. 2015. Effects of a Particular Heptapeptide on the IFN-α-Sensitive CML Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055077
Modern Language Association (MLA)
Yang, Fu-lan…[et al.]. Effects of a Particular Heptapeptide on the IFN-α-Sensitive CML Cells. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055077
American Medical Association (AMA)
Yang, Fu-lan& Chen, Fang-zhi& Wan, Xin-xing& Zhou, Xi& Zhou, Mei-juan& Chen, Han-chun…[et al.]. Effects of a Particular Heptapeptide on the IFN-α-Sensitive CML Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055077
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055077