TLR4NF-κB-Responsive MicroRNAs and Their Potential Target Genes: A Mouse Model of Skeletal Muscle Ischemia-Reperfusion Injury
Joint Authors
Yang, Johnson Chia-Shen
Wu, Shao-Chun
Rau, Cheng-Shyuan
Chen, Yi-Chun
Lu, Tsu-Hsiang
Wu, Yi-Chan
Tzeng, Siou-Ling
Wu, Chia-Jung
Hsieh, Ching-Hua
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
The aim of this study was to profile TLR4/NF-κB-responsive microRNAs (miRNAs) and their potential target genes in the skeletal muscles of mice following ischemia-reperfusion injury.
Methods.
Thigh skeletal muscles of C57BL/6, Tlr4−/−, and NF-κB−/− mice isolated based on femoral artery perfusion were subjected to ischemia for 2 h and reperfusion for 0 h, 4 h, 1 d, and 7 d.
The muscle specimens were analyzed with miRNA arrays.
Immunoprecipitation with an argonaute 2- (Ago2-) specific monoclonal antibody followed by whole genome microarray was performed to identify mRNA associated with the RNA-silencing machinery.
The potential targets of each upregulated miRNA were identified by combined analysis involving the bioinformatics algorithm miRanda and whole genome expression.
Results.
Three TLR4/NF-κB-responsive miRNAs (miR-15a, miR-744, and miR-1196) were significantly upregulated in the muscles following ischemia-reperfusion injury.
The combined in silico and whole genome microarray approaches identified 5, 4, and 20 potential target genes for miR-15a, miR-744, and miR-1196, respectively.
Among the 3 genes (Zbed4, Lrsam1, and Ddx21) regulated by at least 2 of the 3 upregulated miRNAs, Lrsam1 and Ddx21 are known to be associated with the innate immunity pathway.
Conclusions.
This study profiled TLR4/NF-κB-responsive miRNAs and their potential target genes in mouse skeletal muscle subjected to ischemia-reperfusion injury.
American Psychological Association (APA)
Yang, Johnson Chia-Shen& Wu, Shao-Chun& Rau, Cheng-Shyuan& Chen, Yi-Chun& Lu, Tsu-Hsiang& Wu, Yi-Chan…[et al.]. 2015. TLR4NF-κB-Responsive MicroRNAs and Their Potential Target Genes: A Mouse Model of Skeletal Muscle Ischemia-Reperfusion Injury. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055377
Modern Language Association (MLA)
Yang, Johnson Chia-Shen…[et al.]. TLR4NF-κB-Responsive MicroRNAs and Their Potential Target Genes: A Mouse Model of Skeletal Muscle Ischemia-Reperfusion Injury. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1055377
American Medical Association (AMA)
Yang, Johnson Chia-Shen& Wu, Shao-Chun& Rau, Cheng-Shyuan& Chen, Yi-Chun& Lu, Tsu-Hsiang& Wu, Yi-Chan…[et al.]. TLR4NF-κB-Responsive MicroRNAs and Their Potential Target Genes: A Mouse Model of Skeletal Muscle Ischemia-Reperfusion Injury. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055377
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055377