miR-136 Modulates TGF-β1-Induced Proliferation Arrest by Targeting PPP2R2A in Keratinocytes
Joint Authors
Zhang, Dianbao
Wang, Jing
Wang, Zhe
Zhang, Tao
Shi, Ping
Wang, Xiliang
Zhao, Feng
Liu, Xiaoyu
Lin, Xuewen
Pang, Xining
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Keratinocytes proliferation is critical for the capacity to heal wounds and accumulating evidences have proved that dysregulation of microRNAs is involved in proliferation of keratinocytes.
However, the molecular mechanisms remain to be completely elucidated.
Here, we show that miR-136 was significantly decreased by TGF-β1 treatment in HaCaT cells and normal human epidermal keratinocytes (NHEK), and it was a Smad3-dependent manner.
By cell proliferation assay and cell cycle analysis, we found that reintroduction of miR-136 by transfection, as well as PPP2R2A silencing, counteracted TGF-β-induced proliferation arrest in HaCaT cells.
Further, PPP2R2A was verified as a direct target of miR-136 by dual-luciferase reporter assays and Western blotting.
These data suggest that miR-136 may play an important role during TGF-β1-induced proliferation arrest by targeting PPP2R2A in keratinocytes, which might represent a potential target for improving skin wound healing.
American Psychological Association (APA)
Zhang, Dianbao& Wang, Jing& Wang, Zhe& Zhang, Tao& Shi, Ping& Wang, Xiliang…[et al.]. 2015. miR-136 Modulates TGF-β1-Induced Proliferation Arrest by Targeting PPP2R2A in Keratinocytes. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055518
Modern Language Association (MLA)
Zhang, Dianbao…[et al.]. miR-136 Modulates TGF-β1-Induced Proliferation Arrest by Targeting PPP2R2A in Keratinocytes. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055518
American Medical Association (AMA)
Zhang, Dianbao& Wang, Jing& Wang, Zhe& Zhang, Tao& Shi, Ping& Wang, Xiliang…[et al.]. miR-136 Modulates TGF-β1-Induced Proliferation Arrest by Targeting PPP2R2A in Keratinocytes. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055518
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055518