Effect of Luteolin and Apigenin on the Expression of Oct-4, Sox2, and c-Myc in Dental Pulp Cells with In Vitro Culture
Joint Authors
Liu, Lu
Peng, Zhengjun
Xu, Zezhen
Wei, Xi
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Introduction.
Dental pulp cells (DPCs) are promising cell source for dental tissue regeneration.
Recently, small molecules which optimize microenvironment or activate the reprogramming network provide a new way to enhance the pluripotency.
Two promising bioflavonoids luteolin and apigenin were reported to enhance reprogramming efficiency in mouse embryonic fibroblast (MEF).
However, their effect and underlying mechanism in cell fate determination of human DPCs remain unclear.
Methods.
To elucidate the effect of luteolin and apigenin on the cell fate determination of DPCs, we explored the cell proliferation, cell cycle, senescence, apoptosis, expression of pluripotency markers Oct-4, Sox2, and c-Myc, and multilineage differentiation capability of DPCs with luteolin or apigenin treatment.
Results.
We demonstrated that luteolin and apigenin inhibited cell proliferation, arrested DPCs in G2/M and S phase, and upregulated PI value and apoptosis.
Moreover, luteolin and apigenin increased telomerase activity, maintained DPCs in a presenescent state, and activated the expression of Oct-4, Sox2, and c-Myc at a dose- and time-dependent pattern in DPCs even at late passages, albeit repressed lineage-specific differentiation.
Conclusions.
Addition of luteolin and apigenin in the culture medium might provide an effective way to maintain DPCs in an undifferentiated stage and inhibit lineage-specific differentiation.
American Psychological Association (APA)
Liu, Lu& Peng, Zhengjun& Xu, Zezhen& Wei, Xi. 2015. Effect of Luteolin and Apigenin on the Expression of Oct-4, Sox2, and c-Myc in Dental Pulp Cells with In Vitro Culture. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055852
Modern Language Association (MLA)
Liu, Lu…[et al.]. Effect of Luteolin and Apigenin on the Expression of Oct-4, Sox2, and c-Myc in Dental Pulp Cells with In Vitro Culture. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055852
American Medical Association (AMA)
Liu, Lu& Peng, Zhengjun& Xu, Zezhen& Wei, Xi. Effect of Luteolin and Apigenin on the Expression of Oct-4, Sox2, and c-Myc in Dental Pulp Cells with In Vitro Culture. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055852
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055852