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Biological Production of an Integrin αvβ3 Targeting Imaging Probe and Functional Verification
Joint Authors
Ahn, Byeong-Cheol
Senthilkumar, Kalimuthu
Hwang, Mi-Hye
Kim, Jung Eun
Kim, Sang-Yeob
Jeong, Shin Young
Lee, Jaetae
Lee, Sang-Woo
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of the present study is to establish a bacterial clone capable of secreting an integrin αvβ3 targeting probe with bioluminescent and fluorescent activities, and to verify its specific targeting and optical activities using molecular imaging.
A bacterial vector expressing a fusion of secretory Gaussia luciferase (sGluc), mCherry, and RGD (sGluc-mCherry-RGDX3; GCR), and a control vector expressing a fusion of secretory Gaussia luciferase and mCherry (sGluc-mCherry; GC) were constructed.
The GCR and GC proteins were expressed in E.
coli and secreted into the growth medium, which showed an approximately 10-fold higher luciferase activity than the bacterial lysate.
Successful purification of GCR and GC was achieved using the 6X His-tag method.
The GCR protein bound with higher affinity to U87MG cells than CHO cells in confocal microscopy and IVIS imaging, and also showed a high affinity for integrin αvβ3 expressing tumor xenografts in an in vivo animal model.
An E.
coli clone was established to secrete an integrin αvβ3 targeting imaging probe with bioluminescent and fluorescent activities.
The probe was produced feasibly and at low cost, and has shown to be useful for the assessment of angiogenesis in vitro and in vivo.
American Psychological Association (APA)
Hwang, Mi-Hye& Kim, Jung Eun& Kim, Sang-Yeob& Senthilkumar, Kalimuthu& Jeong, Shin Young& Lee, Sang-Woo…[et al.]. 2015. Biological Production of an Integrin αvβ3 Targeting Imaging Probe and Functional Verification. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056367
Modern Language Association (MLA)
Hwang, Mi-Hye…[et al.]. Biological Production of an Integrin αvβ3 Targeting Imaging Probe and Functional Verification. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1056367
American Medical Association (AMA)
Hwang, Mi-Hye& Kim, Jung Eun& Kim, Sang-Yeob& Senthilkumar, Kalimuthu& Jeong, Shin Young& Lee, Sang-Woo…[et al.]. Biological Production of an Integrin αvβ3 Targeting Imaging Probe and Functional Verification. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056367
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056367