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Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells
Joint Authors
Liu, Jun
Fan, Shengnuo
Zhong, Jianbin
Yan, Zhenwen
Xiao, Songhua
Wan, Limei
Chen, Chibang
Zhong, Simin
Liu, Lu
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-25
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Parkinson’s disease (PD) is a common degenerative disease that lacks efficient treatment.
Myelin-associated neurite outgrowth inhibitor A (Nogo-A) is relevant with inhibition of nerve regeneration and may play vital role in pathogenesis of PD.
The study aimed to establish the shRNA expression plasmids of Nogo-A gene and explore the regulatory effects of Nogo-A silencing on the expression of inflammation factor tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) as well as tyrosine hydroxylase (TH) in lipopolysaccharide- (LPS-) stimulated rat PC12 cells.
The results showed that both mRNA and protein levels of Nogo-A in pGenesil-nogoA-shRNA group were downregulated.
The viabilities of PC12 cells decreased with increase of LPS concentrations.
LPS significantly increased the supernatant TNF-alpha and IL-6 concentrations and reduced TH protein expression in PC12 cells, while silencing Nogo-A could block these effects.
These results suggested that LPS can activate PC12 cells to secrete inflammatory cytokines and lower the TH expression, which can be regulated by Nogo-A gene silencing.
Nogo-A silencing might provide new ideas for PD treatment in the future.
American Psychological Association (APA)
Zhong, Jianbin& Fan, Shengnuo& Yan, Zhenwen& Xiao, Songhua& Wan, Limei& Chen, Chibang…[et al.]. 2015. Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1056851
Modern Language Association (MLA)
Zhong, Jianbin…[et al.]. Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1056851
American Medical Association (AMA)
Zhong, Jianbin& Fan, Shengnuo& Yan, Zhenwen& Xiao, Songhua& Wan, Limei& Chen, Chibang…[et al.]. Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1056851
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056851