Impact of Cadmium on Intracellular Zinc Levels in HepG2 Cells: Quantitative Evaluations and Molecular Effects
Joint Authors
Fabbri, M.
Urani, Chiara
Melchioretto, Pasquale
Bruschi, Maurizio
Sacco, Maria Grazia
Gribaldo, Laura
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cadmium is classified as a human carcinogen, and its disturbance in zinc homeostasis has been well established.
However, its extent as well as molecular mechanisms involved in cadmium carcinogenesis has yet to be fully clarified.
To this end, we used the zinc specific probe Zinquin to visualize and to quantitatively evaluate changes in the concentration of labile zinc, in an in vitro model of human hepatic cells (HepG2) exposed to cadmium.
A very large increase (+93%) of intracellular labile zinc, displaced by cadmium from the zinc proteome, was measured when HepG2 were exposed to 10 µM cadmium for 24 hrs.
Microarray expression profiling showed that in cells, featuring an increase of labile zinc after cadmium exposure, one of the top regulated genes is Snail1 (+3.6), which is included in the adherens junction pathway and linked to cancer.
In the same pathway MET, TGF-βR, and two members of the Rho-family GTPase, Rac, and cdc42 all implicated in the loss of adherence features and acquisition of migratory and cancer properties were regulated, as well.
The microRNAs analysis showed a downregulation of miR-34a and miR-200a, both implicated in the epithelial-mesenchymal transition.
These microRNAs results support the role played by zinc in affecting gene expression at the posttranscriptional level.
American Psychological Association (APA)
Urani, Chiara& Melchioretto, Pasquale& Bruschi, Maurizio& Fabbri, M.& Sacco, Maria Grazia& Gribaldo, Laura. 2015. Impact of Cadmium on Intracellular Zinc Levels in HepG2 Cells: Quantitative Evaluations and Molecular Effects. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057353
Modern Language Association (MLA)
Urani, Chiara…[et al.]. Impact of Cadmium on Intracellular Zinc Levels in HepG2 Cells: Quantitative Evaluations and Molecular Effects. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1057353
American Medical Association (AMA)
Urani, Chiara& Melchioretto, Pasquale& Bruschi, Maurizio& Fabbri, M.& Sacco, Maria Grazia& Gribaldo, Laura. Impact of Cadmium on Intracellular Zinc Levels in HepG2 Cells: Quantitative Evaluations and Molecular Effects. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057353
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057353