Hiwi Promotes the Proliferation of Colorectal Cancer Cells via Upregulating Global DNA Methylation
Joint Authors
Yang, Lin
Bi, Lei
Liu, Qingwei
Zhao, Meng
Cao, Bin
Li, Dong
Xiu, Jianjun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Hiwi is well known for its role in stem cell renewal, maintaining the resting stage, and downregulating cell cycle of stem cells via RNA silencing.
And Hiwi overexpression has been recognized in several types of cancers.
In the present study, we examined the Hiwi expression in colorectal cancer (CRC) specimens in both mRNA and protein levels via real-time quantitative PCR, western blot assay, and immunohistochemical staining.
Then we explored the role of Hiwi in the cancer cell proliferation and in the DNA methylation in human CRC Caro-2 and HT-29 cell lines.
Results demonstrated that both mRNA and protein levels of Hiwi were significantly higher in 38 CRC tissues than in 38 peritumor tissues.
Moreover, the Hiwi overexpression with an adenovirus vector significantly promoted the proliferation of Caro-2 and HT-29 cells, associated with significant increase in the global DNA methylation levels.
And the chemical inhibition of DNA methylation significantly restrained such proliferation promotion.
In summary, we confirmed that Hiwi was overexpressed in CRC tissues and that the forced Hiwi overexpression promoted the proliferation and global DNA methylation of CRC cell lines.
Our results imply for the first time that Hiwi promotes the proliferation of CRC cells via promoting global DNA methylation.
American Psychological Association (APA)
Yang, Lin& Bi, Lei& Liu, Qingwei& Zhao, Meng& Cao, Bin& Li, Dong…[et al.]. 2015. Hiwi Promotes the Proliferation of Colorectal Cancer Cells via Upregulating Global DNA Methylation. Disease Markers،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1060928
Modern Language Association (MLA)
Yang, Lin…[et al.]. Hiwi Promotes the Proliferation of Colorectal Cancer Cells via Upregulating Global DNA Methylation. Disease Markers No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1060928
American Medical Association (AMA)
Yang, Lin& Bi, Lei& Liu, Qingwei& Zhao, Meng& Cao, Bin& Li, Dong…[et al.]. Hiwi Promotes the Proliferation of Colorectal Cancer Cells via Upregulating Global DNA Methylation. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1060928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1060928