Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus acutissima Cortex
Joint Authors
Kaneko, Atsushi
Kase, Yoshio
Sekiguchi, Kyoji
Koseki, Junichi
Tsuchiya, Kazuaki
Matsubara, Yosuke
Matsumoto, Takashi
Watanabe, Junko
Mizuhara, Yasuharu
Nishimura, Hiroaki
Maemura, Kazuya
Hattori, Tomohisa
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
Bokusoku (BK) is an extract from the Quercus cortex used in folk medicine for treatment of skin disorders and convergence, and is present in jumihaidokuto, a traditional Japanese medicine that is prescribed for purulent skin diseases like acne vulgaris.
The excess of sebum production induced by androgen is involved in the development of acne.
Our aim is to examine whether BK and its constituents inhibit testosterone metabolism and testosterone-induced sebum synthesis.
Methods.
Measurements of 5α-reductase activity and lipogenesis were performed using rat liver microsomes and hamster sebocytes, respectively.
Results.
BK dose-dependently reduced the conversion of testosterone to a more active androgen, dihydrotestosterone in a 5α-reductase enzymatic reaction.
Twenty polyphenols in BK categorized as gallotannin, ellagitannin, and flavonoid were identified by LC-MS/MS.
Nine polyphenols with gallate group, tetragalloyl glucose, pentagalloyl glucose, eugeniin, 1-desgalloyl eugeniin, casuarinin, castalagin, stenophyllanin C, (−)-epicatechin gallate, and (−)-epigallocatechin gallate, inhibited testosterone metabolism.
In particular, pentagalloyl glucose showed the strongest activity.
BK and pentagalloyl glucose suppressed testosterone-induced lipogenesis, whereas they weakly inhibited the lipogenic action of insulin.
Conclusions.
BK inhibited androgen-related pathogenesis of acne, testosterone conversion, and sebum synthesis, partially through 5α-reductase inhibition, and has potential to be a useful agent in the therapeutic strategy of acne.
American Psychological Association (APA)
Koseki, Junichi& Matsumoto, Takashi& Matsubara, Yosuke& Tsuchiya, Kazuaki& Mizuhara, Yasuharu& Sekiguchi, Kyoji…[et al.]. 2015. Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus acutissima Cortex. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063684
Modern Language Association (MLA)
Koseki, Junichi…[et al.]. Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus acutissima Cortex. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063684
American Medical Association (AMA)
Koseki, Junichi& Matsumoto, Takashi& Matsubara, Yosuke& Tsuchiya, Kazuaki& Mizuhara, Yasuharu& Sekiguchi, Kyoji…[et al.]. Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus acutissima Cortex. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063684
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063684