Ghrelin’s Effects on Proinflammatory Cytokine Mediated Apoptosis and Their Impact on β-Cell Functionality
Joint Authors
Segundo, Carmen
Diaz-Ganete, Antonia
Baena-Nieto, Gloria
Lomas-Romero, Isabel M.
Lopez-Acosta, Jose Francisco
Cozar-Castellano, Irene
Medina, Francisco
Lechuga-Sancho, Alfonso M.
Source
International Journal of Endocrinology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Ghrelin is a peptidic hormone, which stimulates cell proliferation and inhibits apoptosis in several tissues, including pancreas.
In preclinical stage of type 1 diabetes, proinflammatory cytokines generate a destructive environment for β-cells known as insulitis, which results in loss of β-cell mass and impaired insulin secretion, leading to diabetes.
Our aim was to demonstrate that ghrelin could preserve β-cell viability, turnover rate, and insulin secretion acting as a counter balance of cytokines.
In the present work we reproduced proinflammatory milieu found in insulitis stage by treating murine cell line INS-1E and rat islets with a cytokine cocktail including IL-1β, IFNγ, and TNFα and/or ghrelin.
Several proteins involved in survival pathways (ERK 1/2 and Akt/PKB) and apoptosis (caspases and Bcl-2 protein family and endoplasmic reticulum stress markers) as well as insulin secretion were analyzed.
Our results show that ghrelin alone has no remarkable effects on β-cells in basal conditions, but interestingly it activates cell survival pathways, downregulates apoptotic mediators and endoplasmic reticulum stress, and restores insulin secretion in response to glucose when beta-cells are cytokine-exposed.
These data suggest a potential role of ghrelin in preventing or slowing down the transition from a preclinical to clinically established diabetes by ameliorating the effects of insulitis on β-cells.
American Psychological Association (APA)
Diaz-Ganete, Antonia& Baena-Nieto, Gloria& Lomas-Romero, Isabel M.& Lopez-Acosta, Jose Francisco& Cozar-Castellano, Irene& Medina, Francisco…[et al.]. 2015. Ghrelin’s Effects on Proinflammatory Cytokine Mediated Apoptosis and Their Impact on β-Cell Functionality. International Journal of Endocrinology،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1065599
Modern Language Association (MLA)
Diaz-Ganete, Antonia…[et al.]. Ghrelin’s Effects on Proinflammatory Cytokine Mediated Apoptosis and Their Impact on β-Cell Functionality. International Journal of Endocrinology No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1065599
American Medical Association (AMA)
Diaz-Ganete, Antonia& Baena-Nieto, Gloria& Lomas-Romero, Isabel M.& Lopez-Acosta, Jose Francisco& Cozar-Castellano, Irene& Medina, Francisco…[et al.]. Ghrelin’s Effects on Proinflammatory Cytokine Mediated Apoptosis and Their Impact on β-Cell Functionality. International Journal of Endocrinology. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1065599
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065599