Inhibition of TNF-α Reverses the Pathological Resorption Pit Profile of Osteoclasts from Patients with Acute Charcot Osteoarthropathy
Joint Authors
Petrova, Nina L.
Petrov, Peter K.
Edmonds, Michael E.
Shanahan, Catherine M.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We hypothesised that tumour necrosis factor-α (TNF-α) may enhance receptor activator of nuclear factor-κβ ligand- (RANKL-) mediated osteoclastogenesis in acute Charcot osteoarthropathy.
Peripheral blood monocytes were isolated from 10 acute Charcot patients, 8 diabetic patients, and 9 healthy control subjects and cultured in vitro on plastic and bone discs.
Osteoclast formation and resorption were assessed after treatment with (1) macrophage-colony stimulating factor (M-CSF) and RANKL and (2) M-CSF, RANKL, and neutralising antibody to TNF-α (anti-TNF-α).
Resorption was measured on the surface of bone discs by image analysis and under the surface using surface profilometry.
Although osteoclast formation was similar in M-CSF + RANKL-treated cultures between the groups (p>0.05), there was a significant increase in the area of resorption on the surface (p<0.01) and under the surface (p<0.01) in Charcot patients compared with diabetic patients and control subjects.
The addition of anti-TNF-α resulted in a significant reduction in the area of resorption on the surface (p<0.05) and under the surface (p<0.05) only in Charcot patients as well as a normalisation of the aberrant erosion profile.
We conclude that TNF-α modulates RANKL-mediated osteoclastic resorption in vitro in patients with acute Charcot osteoarthropathy.
American Psychological Association (APA)
Petrova, Nina L.& Petrov, Peter K.& Edmonds, Michael E.& Shanahan, Catherine M.. 2015. Inhibition of TNF-α Reverses the Pathological Resorption Pit Profile of Osteoclasts from Patients with Acute Charcot Osteoarthropathy. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068027
Modern Language Association (MLA)
Petrova, Nina L.…[et al.]. Inhibition of TNF-α Reverses the Pathological Resorption Pit Profile of Osteoclasts from Patients with Acute Charcot Osteoarthropathy. Journal of Diabetes Research No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1068027
American Medical Association (AMA)
Petrova, Nina L.& Petrov, Peter K.& Edmonds, Michael E.& Shanahan, Catherine M.. Inhibition of TNF-α Reverses the Pathological Resorption Pit Profile of Osteoclasts from Patients with Acute Charcot Osteoarthropathy. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068027
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068027