Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation
Joint Authors
Dory, Daniel
Le Moigne, Vincent
Cariolet, Roland
Béven, Véronique
Keranflec’h, André
Jestin, André
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-25
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
DNA vaccination has been developed in the last two decades in human and animal species as a promising alternative to conventional vaccination.
It consists in the injection, in the muscle, for example, of plasmid DNA encoding the vaccinating polypeptide.
Electroporation which forces the entrance of the plasmid DNA in cells at the injection point has been described as a powerful and promising strategy to enhance DNA vaccine efficacy.
Due to the fact that the vaccine is composed of DNA, close attention on the fate of the plasmid DNA upon vaccination has to be taken into account, especially at the injection point.
To perform such studies, the muscle injection point has to be precisely recovered and collected several weeks after injection.
This is even more difficult for large and growing animals.
A technique has been developed to localize precisely and collect efficiently the muscle injection points in growing piglets 6 weeks after DNA vaccination accompanied or not by electroporation.
Electroporation did not significantly increase the level of remaining plasmids compared to nonelectroporated piglets, and, in all the cases, the levels were below the limit recommended by the FDA to research integration events of plasmid DNA into the host DNA.
American Psychological Association (APA)
Dory, Daniel& Le Moigne, Vincent& Cariolet, Roland& Béven, Véronique& Keranflec’h, André& Jestin, André. 2015. Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1068442
Modern Language Association (MLA)
Dory, Daniel…[et al.]. Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation. Journal of Immunology Research No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1068442
American Medical Association (AMA)
Dory, Daniel& Le Moigne, Vincent& Cariolet, Roland& Béven, Véronique& Keranflec’h, André& Jestin, André. Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1068442
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068442