Conjugation of E. coli O157:H7 Antibody to CdSeZnS Quantum Dots
Joint Authors
Vo, N. T.
Ngo, H. D.
Vu, D. L.
Duong, A. P.
Lam, Q. V.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The conjugation of antibody to semiconductor quantum dots plays a very important role in many applications such as bioimaging, biomarking, and biosensing.
In this research, we present some results of highly luminescent core/shell structure CdSe/ZnS on which the E.
coli antibody was conjugated.
The CdSe core was synthesized successfully with chemical “green” method.
For biological applications, the capping surfactant, trioctylphosphine oxide, was substituted by a new one, mercaptopropionic acid (MPA), before the antibody attachment step.
Finally, the E.
coli antibody was attached to quantum dots CdSe/ZnS.
Morphology, structure, and optical properties were investigated with PL, UV-Vis, TEM, and XRD methods.
The successful ligand substitution and antibody attachment were confirmed by zeta potential measurement, FTIR spectroscopy, and TEM.
The results showed quantum dots size of 2.3 nm, uniform distribution, and high luminescence.
CdSe/ZnS core/shell structure had better stability and enhanced the luminescence efficiency up to threefold compared with the core CdSe.
MPA ligand shifted the initial hydrophobic quantum dots to hydrophilic ones, which helped to dissolve them in organic solvents and attach the antibody.
American Psychological Association (APA)
Vo, N. T.& Ngo, H. D.& Vu, D. L.& Duong, A. P.& Lam, Q. V.. 2015. Conjugation of E. coli O157:H7 Antibody to CdSeZnS Quantum Dots. Journal of Nanomaterials،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1068869
Modern Language Association (MLA)
Vo, N. T.…[et al.]. Conjugation of E. coli O157:H7 Antibody to CdSeZnS Quantum Dots. Journal of Nanomaterials No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1068869
American Medical Association (AMA)
Vo, N. T.& Ngo, H. D.& Vu, D. L.& Duong, A. P.& Lam, Q. V.. Conjugation of E. coli O157:H7 Antibody to CdSeZnS Quantum Dots. Journal of Nanomaterials. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1068869
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068869