Caffeine Mitigates Lung Inflammation Induced by Ischemia-Reperfusion of Lower Limbs in Rats
Joint Authors
Kao, Ming-Chang
Huang, Chun-Jen
Chou, Wei-Chi
Yue, Chung-Tai
Tsai, Pei-Shan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Reperfusion of ischemic limbs can induce inflammation and subsequently cause acute lung injury.
Caffeine, a widely used psychostimulant, possesses potent anti-inflammatory capacity.
We elucidated whether caffeine can mitigate lung inflammation caused by ischemia-reperfusion (IR) of the lower limbs.
Adult male Sprague-Dawley rats were randomly allocated to receive IR, IR plus caffeine (IR + Caf group), sham-operation (Sham), or sham plus caffeine ( n = 12 in each group).
To induce IR, lower limbs were bilaterally tied by rubber bands high around each thigh for 3 hours followed by reperfusion for 3 hours.
Caffeine (50 mg/kg, intraperitoneal injection) was administered immediately after reperfusion.
Our histological assay data revealed characteristics of severe lung inflammation in the IR group and mild to moderate characteristic of lung inflammation in the IR + Caf group.
Total cells number and protein concentration in bronchoalveolar lavage fluid of the IR group were significantly higher than those of the IR + Caf group ( P < 0.001 and P = 0 .
008 , resp.).
Similarly, pulmonary concentrations of inflammatory mediators (tumor necrosis factor-α, interleukin-1β, and macrophage inflammatory protein-2) and pulmonary myeloperoxidase activity of the IR group were significantly higher than those of the IR + Caf group (all P < 0.05 ).
These data clearly demonstrate that caffeine could mitigate lung inflammation induced by ischemia-reperfusion of the lower limbs.
American Psychological Association (APA)
Chou, Wei-Chi& Kao, Ming-Chang& Yue, Chung-Tai& Tsai, Pei-Shan& Huang, Chun-Jen. 2015. Caffeine Mitigates Lung Inflammation Induced by Ischemia-Reperfusion of Lower Limbs in Rats. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1072268
Modern Language Association (MLA)
Chou, Wei-Chi…[et al.]. Caffeine Mitigates Lung Inflammation Induced by Ischemia-Reperfusion of Lower Limbs in Rats. Mediators of Inflammation No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1072268
American Medical Association (AMA)
Chou, Wei-Chi& Kao, Ming-Chang& Yue, Chung-Tai& Tsai, Pei-Shan& Huang, Chun-Jen. Caffeine Mitigates Lung Inflammation Induced by Ischemia-Reperfusion of Lower Limbs in Rats. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1072268
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072268