Withaferin A Inhibits Nuclear Factor-κB-Dependent Pro-Inflammatory and Stress Response Pathways in the Astrocytes
Joint Authors
Martorana, Francesca
Guidotti, Giulia
Brambilla, Liliana
Rossi, Daniela
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Several lines of evidence suggest that astrocytes play a key role in modulating the immune responses of the central nervous system (CNS) to infections, injuries, or pathologies.
Yet, their contribution to these processes remains mostly elusive.
Astroglia are endowed with a wide range of toll-like receptors (TLR) by which they can sense infectious agents as well as endogenous danger signals released by damaged cells.
Here we demonstrate that the activation of astrocytic TLR4 by bacterial lipopolysaccharide (LPS) challenge can promote nuclear factor κB (NF-κB)-dependent induction of pro-inflammatory and stress response mediators, particularly Tumor Necrosis Factor α (TNFα), cyclooxygenase 2 (COX-2), and inducible nitric oxide synthase (iNOS).
Since the steroid lactone Withaferin A was described to inhibit NF-κB activity in different cell types, we next determined the impact of this natural compound towards the identified astrocytic signalling pathway.
Innate immune activation was induced by stimulation of the LPS/TLR4 axis in spinal cord astrocytes.
We provide evidence that both pre-treating and post-treating the cells with Withaferin A attenuate astrocytic NF-κB activity as well as the consequent production of TNFα, COX-2, and iNOS induced by stimulation of the LPS/TLR4 pathway.
This study suggests that Withaferin A may be an eligible candidate for the treatment of neuroinflammatory and stress conditions characterized by an important astrocytic input.
American Psychological Association (APA)
Martorana, Francesca& Guidotti, Giulia& Brambilla, Liliana& Rossi, Daniela. 2015. Withaferin A Inhibits Nuclear Factor-κB-Dependent Pro-Inflammatory and Stress Response Pathways in the Astrocytes. Neural Plasticity،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075332
Modern Language Association (MLA)
Martorana, Francesca…[et al.]. Withaferin A Inhibits Nuclear Factor-κB-Dependent Pro-Inflammatory and Stress Response Pathways in the Astrocytes. Neural Plasticity No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1075332
American Medical Association (AMA)
Martorana, Francesca& Guidotti, Giulia& Brambilla, Liliana& Rossi, Daniela. Withaferin A Inhibits Nuclear Factor-κB-Dependent Pro-Inflammatory and Stress Response Pathways in the Astrocytes. Neural Plasticity. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075332
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075332