Myostatin Activates the Ubiquitin-Proteasome and Autophagy-Lysosome Systems Contributing to Muscle Wasting in Chronic Kidney Disease
Joint Authors
Huang, Ren-Hua
Zhang, Zhi-Hua
Lin, Xin
Wang, Dongtao
Yang, Yajun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-13
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Our evidence demonstrated that CKD upregulated the expression of myostatin, TNF-α, and p-IkBa and downregulated the phosphorylation of PI3K, Akt, and FoxO3a, which were also associated with protein degradation and muscle atrophy.
The autophagosome formation and protein expression of autophagy-related genes were increased in muscle of CKD rats.
The mRNA level and protein expression of MAFbx and MuRF-1 were also upregulated in CKD rats, as well as proteasome activity of 26S.
Moreover, activation of myostatin elicited by TNF-α induces C2C12 myotube atrophy via upregulating the expression of autophagy-related genes, including MAFbx and MuRF1 and proteasome subunits.
Inactivation of FoxO3a triggered by PI3K inhibitor LY294002 prevented the myostatin-induced increase of expression of MuRF1, MAFbx, and LC3-II protein in C2C12 myotubes.
The findings were further consolidated by using siRNA interference and overexpression of myostatin.
Additionally, expression of myostatin was activated by TNF-α via a NF-κB dependent pathway in C2C12 myotubes, while inhibition of NF-κB activity suppressed myostatin and improved myotube atrophy.
Collectively, myostatin mediated CKD-induced muscle catabolism via coordinate activation of the autophagy and the ubiquitin-proteasome systems.
American Psychological Association (APA)
Wang, Dongtao& Yang, Yajun& Huang, Ren-Hua& Zhang, Zhi-Hua& Lin, Xin. 2015. Myostatin Activates the Ubiquitin-Proteasome and Autophagy-Lysosome Systems Contributing to Muscle Wasting in Chronic Kidney Disease. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-18.
https://search.emarefa.net/detail/BIM-1075736
Modern Language Association (MLA)
Wang, Dongtao…[et al.]. Myostatin Activates the Ubiquitin-Proteasome and Autophagy-Lysosome Systems Contributing to Muscle Wasting in Chronic Kidney Disease. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-18.
https://search.emarefa.net/detail/BIM-1075736
American Medical Association (AMA)
Wang, Dongtao& Yang, Yajun& Huang, Ren-Hua& Zhang, Zhi-Hua& Lin, Xin. Myostatin Activates the Ubiquitin-Proteasome and Autophagy-Lysosome Systems Contributing to Muscle Wasting in Chronic Kidney Disease. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-18.
https://search.emarefa.net/detail/BIM-1075736
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075736