Modulating Mesenchymal Stem Cell Behavior Using Human Hair Keratin-Coated Surfaces
Joint Authors
Ng, Kee Woei
Hartrianti, Pietradewi
Ling, Ling
Goh, Lyn Mei Ming
Ow, Kok Seng Amos
Samsonraj, Rebekah Margaret
Sow, Wan Ting
Wang, Shuai
Nurcombe, Victor
Cool, Simon M.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-01
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Human mesenchymal stem cells (hMSCs) have shown great potential for therapeutic purposes.
However, the low frequencies of hMSCs in the body and difficulties in expanding their numbers in vitro have limited their clinical use.
In order to develop an alternative strategy for the expansion of hMSCs in vitro, we coated tissue culture polystyrene with keratins extracted from human hair and studied the behavior of cells from 2 donors on these surfaces.
The coating resulted in a homogeneous distribution of nanosized keratin globules possessing significant hydrophilicity.
Results from cell attachment assays demonstrated that keratin-coated surfaces were able to moderate donor-to-donor variability when compared with noncoated tissue culture polystyrene.
STRO-1 expression was either sustained or enhanced on hMSCs cultured on keratin-coated surfaces.
This translated into significant increases in the colony-forming efficiencies of both hMSC populations, when the cells were serially passaged.
Human hair keratins are abundant and might constitute a feasible replacement for other biomaterials that are of animal origin.
In addition, our results suggest that hair keratins may be effective in moderating the microenvironment sufficiently to enrich hMSCs with high colony-forming efficiency ex vivo, for clinical applications.
American Psychological Association (APA)
Hartrianti, Pietradewi& Ling, Ling& Goh, Lyn Mei Ming& Ow, Kok Seng Amos& Samsonraj, Rebekah Margaret& Sow, Wan Ting…[et al.]. 2015. Modulating Mesenchymal Stem Cell Behavior Using Human Hair Keratin-Coated Surfaces. Stem Cells International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1076340
Modern Language Association (MLA)
Hartrianti, Pietradewi…[et al.]. Modulating Mesenchymal Stem Cell Behavior Using Human Hair Keratin-Coated Surfaces. Stem Cells International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1076340
American Medical Association (AMA)
Hartrianti, Pietradewi& Ling, Ling& Goh, Lyn Mei Ming& Ow, Kok Seng Amos& Samsonraj, Rebekah Margaret& Sow, Wan Ting…[et al.]. Modulating Mesenchymal Stem Cell Behavior Using Human Hair Keratin-Coated Surfaces. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1076340
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076340